Single Stranded DNA-Binding Protein, ssCRE-BP/Purα、in Rat Lung and Its Increase in Allergic Airway Inflammation

• Wei Er-Qing

  Present address: Department of Pharmacology, Zhejiang Medical University

• Irie Yasuyuki

  Department of Pharmacology, Osaka University Medical School

• Kuo Che-Hui

  Department of Pharmacology, Osaka University Medical School

• Ding Yun

  Department of Pharmacology, Osaka University Medical School

• Niu San-Yong

  Department of Pharmacology, Osaka University Medical School

• Do Eunju

  Department of Pharmacology, Osaka University Medical School

• Miki Naomasa

  Department of Pharmacology, Osaka University Medical School

書誌事項

タイトル別名

• A Single Stranded DNA-Binding Protein, ssCRE-BP/Pur.ALPHA., in Rat Lung and Its Increase in Allergic Airway Inflammation.
• Single Stranded DNA-Binding Protein ssC
• A Single Stranded DNA-Binding Protein, ssCRE-BP/Purα, in Rat Lung and Its Increase in Allergic Airway Inflammation

抄録

ssCRE-BP/Purα is a single stranded DNA-binding protein and may be involved in gene replication and transcription and in the development of morphine dependence. We found a ssCRE-BP/Purα (45 kDa) in rat lung that was larger than those (40 kDa) identified in rat and mouse brains and mouse lung. Immunohistochemistry showed that ssCRE-BP/Purα is primarily distributed in the lung epithelium. As allergic inflammation induces various gene expressions, we investigated the changes of Purα during airway inflammation. Ovalbumin-sensitized rats were used for inducing allergic airway inflammation. The expression and DNA-binding activity of 45-kDa ssCRE-BP/Purα were significantly increased in the sensitized rat lungs 24 hr after antigen challenge, but not in those of rats nonsensitized or sensitized with ovalbumin and challenged with saline. Immunohistochemistry and in situ hybridization demonstrated that the vascular endothelial cells and numerous infiltrated eosinophils around the airways were stained with anti-Purα antibody. These data suggest that rat lung and the eosinophils contain a 45-kDa ssCRE-BP/Purα that is increased when airway inflammation occurs.

収録刊行物

• Jpn.J.Pharmacol.

  Jpn.J.Pharmacol. 78 (4), 419-427, 1998

  公益社団法人 日本薬理学会

キーワード

• Single stranded DNA-binding protein
• ssCRE-BP/Purα
• Allergic airway inflammation
• Eosinophil
• Lung

詳細情報

• CRID: 1390282679263816960
• NII論文ID: 10008681621
• NII書誌ID: AA00691188
• DOI: 10.1254/jjp.78.419
• COI: 1:CAS:528:DyaK1MXhvFWmtg%3D%3D
• ISSN: 13473506
• ISSN: 00215198
• NDL書誌ID: 4629546
• PubMed: 9920198
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4629546
• Web Site: https://api.elsevier.com/content/article/PII:S0021519819310054?httpAccept=text/xml
• Web Site: https://api.elsevier.com/content/article/PII:S0021519819310054?httpAccept=text/plain
• Web Site: https://www.jstage.jst.go.jp/article/jjp/78/4/78_4_419/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/1999116670
• 本文言語コード: en
• データソース種別:
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  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
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