The Expression of Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) and Its Regulation by Ovarian Steroids in Rat Uterine Stromal Cells
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The effects of ovarian steroids on the expression of granulocyte-macrophage colony-stimulating factor (GM-CSF) in rat uterus were examined. Intense expression of GM-CSF mRNA was dispersedly located in the endometrial-myometrial junction and stroma of the uterus. GM-CSF was immunohistochemically localized in stromal cells and luminal epithelium. Ovariectomy significantly reduced the appearance of GM-CSF-mRNA-positive cells and the levels of expression for GM-CSF mRNA in the whole uterus, whereas treatment with 17β-estradiol (E<SUB>2</SUB>) or a combination of E<SUB>2</SUB> and progesterone (P<SUB>4</SUB>) for 5 days on ovariectomized animals recruited GM-CSF-mRNA-positive cells and stimulated its expression. The combined treatment with E<SUB>2</SUB> and P<SUB>4</SUB> also stimulates the expression for GM-CSF mRNA and the production of immunoreactive GM-CSF in the stromal tissues. These data suggest that the expression of GM-CSF in uterine stromal cells is partially regulated by ovarian steroids.
- The Japanese Journal of Pharmacology
The Japanese Journal of Pharmacology 79(2), 257-262, 1999-02-01
The Japanese Pharmacological Society