Chronic Hepatitis in Interferon-γ Transgenic Mice Is Associated With Elevated CPP32-Like Activity and Interleukin-1β-Converting Enzyme Activity Suppression

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Author(s)

Abstract

Interferon-γ (IFN-γ) transgenic mice strongly express IFN-γ in the liver and develop chronic hepatitis. Furthermore, hepatocyte apoptosis was shown by the TdT-mediated dUTP-biotin nick endlabel- ing method. In the present study, interleukin-1β-converting enzyme (ICE) and CPP32-like protease activ- ities in the liver of IFN-γ transgenic mice were measured, using the synthetic substrates Ac-YVAD-MCA and Ac-DEVD-MCA. Plasma aspartate aminotransferase and alanine aminotransferase activities as well as CPP32-like activity were significantly elevated, while ICE activity was significantly reduced. The addition of the ICE inhibitor Ac-YVAD-CHO to IFN-γ transgenic mouse liver cell cytosol had no effect on the CPP32 activity, in contrast to a CPP32 inhibitor. The present results indicate that chronic hepatitis in the IFN-γ transgenic mouse is associated with a decrease in ICE and induction of CPP32-like activity.

Journal

  • The Japanese Journal of Pharmacology

    The Japanese Journal of Pharmacology 79(3), 289-294, 1999-03-01

    The Japanese Pharmacological Society

References:  22

Codes

  • NII Article ID (NAID)
    10008682689
  • NII NACSIS-CAT ID (NCID)
    AA00691188
  • Text Lang
    ENG
  • Article Type
    REV
  • ISSN
    00215198
  • NDL Article ID
    4699119
  • NDL Source Classification
    ZS51(科学技術--薬学)
  • NDL Call No.
    Z53-D199
  • Data Source
    CJP  NDL  J-STAGE 
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