Enhanced EXpression of The LDH-A Gene after Gravity-changing Stress in Human RSa CellS

この論文にアクセスする

この論文をさがす

著者

抄録

A major issue in radiation and space biology is whether gene expression levels are altered in cells exposed to gravity-changing stress. In the present study, genes up- or down-regulated in radiation-sensitive human RSa cells cultured under gravity-changing conditions, were identified using a PCR-based mRNA differential display method. Exposure of cells to gravity-changing stress was performed by free-fall with a drop-shaft facility or by an airplane-conducted parabolic flight. Among the candidates for gravity-changing stress-responsive genes obtained by the differential display analysis, the lactate dehydrogenase A gene (<i>LDH-A</i>) was confirmed by Northern blotting analysis to exhibit increased expression levels. The gravity-changing stress consisted of a combination of microgravity and hypergravity. However, exposure of the cells to hypergravity produced by centrifuge only slightly affected the LDH-A mRNA expression. Thus, <i>LDH-A</i> was found to be a candidate for the genes which play a role in the cellular response to gravity-changing stress, and mainly to microgravity.

収録刊行物

  • 宇宙生物科学 = Biological sciences in space

    宇宙生物科学 = Biological sciences in space 16(1), 12-17, 2002-03-01

    日本宇宙生物科学会

参考文献:  30件中 1-30件 を表示

被引用文献:  1件中 1-1件 を表示

各種コード

  • NII論文ID(NAID)
    10008690361
  • NII書誌ID(NCID)
    AN10164806
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    09149201
  • NDL 記事登録ID
    6193784
  • NDL 雑誌分類
    ZR1(科学技術--生物学) // ZM33(科学技術--宇宙科学)
  • NDL 請求記号
    Z18-2504
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
ページトップへ