Detection of Astrovirus RNA from Sewage Works, Seawater and Native Oysters Samples in Chiba City, Japan Using Reverse Transcription-Polymerase Chain Reaction

• YOKOI Hajime

  Chiba City Institute of Health and Environment

• KITAHASHI Tomoko

  Chiba City Institute of Health and Environment

• TANAKA Toshimitsu

  Chiba City Inspection Center of Food Hygiene

• UTAGAWA Etsuko

  National Institute of Infectious Diseases

Bibliographic Information

Other Title

• 千葉市における下水処理施設, 海水, カキからのアストロウイルス遺伝子の検出
• チバシ ニ オケル ゲスイ ショリ シセツ カイスイ カキ カラ ノ アストロウイルス イデンシ ノ ケンシュツ

Abstract

Through a year from April, 1999 to March, 2000, 20 samples, which consisted of raw sewage (2), chlorine-treated sewage (2), seawater (10) and naturally grown oysters (6), were collected monthly both from the sewage works at Mihama-ku, Chiba City and at a yacht harbor in Chiba City Bay, Japan. Astrovirus RNA were detected by reverse transcription-polymerase chain reaction (RT-PCR) and was typed by direct sequencing.<BR>Astrovirus positive products were detected from 9 samples (raw sewage; 1/2, chlorine-treated sewage; 2/2, seawater; 5/10 and oysters; 1/6) collected in April, 1999. In May, positive products were detected from 4 samples (raw sewage; 2/2 and seawater; 2/10). In June, only 1 positive product was detected from raw sewage. The number of positive samples showed a tendency to decrease and no positive products were detected from samples collected in July, 1999 to January, 2000. After that period, positive products were again detected from 3 samples (raw sewage; 1/2, chlorine-treated sewage; 2/2) collected in February, 2000. In March, the number of positive samples showed the peak and positive products were detected from 12 samples (raw sewage; 2/2, chlorine-treated sewage; 2/2, seawater; 7/10 and oysters: 1/6).<BR>Astrovirus positive products detected in April, May, June, July, 1999 and February, 2000 were classified into type 1 or 2 by sequencing, whereas in March, 2000 were type 1, 2, 3, 6 and 7.

Journal

• Kansenshogaku Zasshi

  Kansenshogaku Zasshi 75 (4), 263-269, 2001

  The Japanese Association for Infectious Diseases

Keywords

• astrovirus
• sewage
• seawater
• oyster
• PCR

Details

• CRID: 1390001205049981184
• NII Article ID: 130004330963
• NII Article ID: 10011297375
• NII Article ID: 10008702943
• NII Book ID: AN00047715
• DOI: 10.11150/kansenshogakuzasshi1970.75.263
• ISSN: 1884569X
• ISSN: 03875911
• NDL BIB ID: 5740052
• PubMed: 11357315
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I5740052
• Web Site: https://search.jamas.or.jp/link/ui/2001211259
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• Abstract License Flag: Disallowed