Evaluation of Immunochromatography Method for Rapid Detection of Shigatoxin

DOI Web Site PubMed 3 Citations 13 References

Bibliographic Information

Other Title
  • イムノクロマト法を用いたシガ毒素の迅速検出法の検討
  • イムノクロマトホウ オ モチイタ シガ ドクソ ノ ジンソク ケンシュツホウ ノ ケントウ

Search this article

Abstract

The Shigatoxin detection kit based on the immunochromatography system is commercially available. To obtain the identification result rapidly, we devised the improved method (ICG-Imp) replaced to an original method (ICG). Modification provided that Shigatoxins extracted directly from the strains grown on TSI medium without centrifugation. ICG-Imp was compared with ICG, RPLA and PCR.<BR>Comparing with RPLA, the sensitivity, specificity, and concordance rate of Shigatoxin 1 showed 77.5, 100 and 90.5%, respectively on ICG, 93.8, 100 and 97.4%, respectively on ICG-Imp, and 100, 99.1 and 99.5%, respectively on PCR. On the other hand, the patterns of Shigatoxin 2 showed 95.3, 100 and 96.3%, respectively on ICG, 100, 100, and 100%, respectively on ICG-Imp, and 100, 100 and 100%, respectively on PCR.<BR>The time required from TSI medium to the final result are 24h, 30-60 min, 48h, and 6h, respectively by ICG, ICG-Imp, RPLA, and PCR. It seems that the ICG-Imp is recommended for the identification by means of the accuracy and rapidness.

Journal

  • Kansenshogaku Zasshi

    Kansenshogaku Zasshi 75 (4), 270-275, 2001

    The Japanese Association for Infectious Diseases

Citations (3)*help

See more

References(13)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top