Detection of Bactericidal Antibody in the Breast Milk of a Mother Infected with Enterohemorrhagic <I>Escherichia coli</I> O157: H7

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  • ADACHI Eriko
    Department of Infectious Diseases Research, National Children's Medical Research Center
  • TANAKA Hirohiko
    Department of Obstetrics and Gynecology, Mie Prefectural Shima Hospital
  • TOYODA Nagayasu
    Department of Obstetrics and Gynecology, Mie University School of Medicine
  • TAKEDA Tae
    Department of Infectious Diseases Research, National Children's Medical Research Center

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Other Title
  • 腸管出血性大腸菌O157: H7に感染した妊婦の血清および母乳中に検出される抗体
  • チョウカン シュッケツセイ ダイチョウキン O157 H7 ニ カンセン シタ ニンプ ノ ケッセイ オヨビ ボニュウ チュウ ニ ケンシュツ サレル コウタイ
  • Detection of Bactericidal Antibody in the Breast Milk of a Mother Infected with Enterohemorrhagic Escherichia coli O157: H7

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Abstract

A21years-old pregnant woman developed diarrhea, fresh bloody stoolsand abdominal pain on April 6th1997 at 32 weeks of gestation, and was admitted to the hospital on April 11th. The stool culture on admission was positive for enterohemorrhagic Escherichia coli (EHEC) O157: H7 (Stxl and 2). Clinical laboratory data during admission showed only slight elevation of β-microglobulin and Nacetyl glucosaminidase in the urine, and no neurological or hemolytic symptoms were seen. After the antibiotic and lactobacillus administration, all her symptoms were relieved and no abnormal findings in pregnancy were observed. She delivered a baby girl normally on May 30 th. Serum (between 41 and 120days from the onset) and milk (between 4 and 64 days post partum) samples from the mother, and serum (64 days of age) from a baby and cord blood were obtainedto monitor the immune status against EHEC O157: H7and against Shiga toxins (Stx). Anti-E. coli O157LPS antibodies (IgA, G and M) were assayed by the ELISA method. Neutralizing anti-Stx antibodies were measured by using ACHN cell cytotoxicity assay.<BR>In the colostrum and mature milk, high levels of IgA and IgM, and no IgG antibodies against EHEC O157 LPS were detected. In one of the control colostrum samplesobtained from4healthy mothers IgA antibody against EHEC O157 LPS was detected. To assess the potency of protection against EHEC O157: H7by the breast milk, we monitored it by the bactericidal activity for the organism under complement-coincubation experiment, and by the neutralization test for the Stx cytotoxicity. As a result, breast milk samples (both colostrum and mature milk) from a patient were demonstrated to kill the organisms. One of 4 healthy milk samples, showed bactericidal activity though it was negative in O157-LPS antibody. This bactericidal activity seen in one healthy colostrum is possibly due to a nonspecific reaction caused by non-0157E. coli infection. From these observations, it was suggested that the bactericidal activity was due to the IgM class antibody against EHEC O157: H7. However, the neutralizing antibody against Stxl and 2could not be detected in any sample.<BR>EHEC infection at late gestation did not cause adverse effects to afetus, and breastfeeding may have advantage for the protection of a baby against EHEC infection.

Journal

  • Kansenshogaku Zasshi

    Kansenshogaku Zasshi 73 (5), 451-456, 1999

    The Japanese Association for Infectious Diseases

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