Evaluation of a Synthetic Peptide Based Species Specific EIA Kit for Detection of Antibodies to Chlamydia trachomatis with Clinical Specimens

• OUCHI Kazunobu

  Department of Pediatrics, Saiseikai Shimonoseki General Hospital

• HASEGAWA Keiko

  Department of Pediatrics, Saiseikai Shimonoseki General Hospital

• MAKI Ryuji

  Department of Pediatrics, Saiseikai Shimonoseki General Hospital

• MORIOKA Hitoshi

  Department of Obstetrics and Gynecology, Saiseikai Shimonoseki General Hospital

• MATSUSHIMA Hiroshi

  Department of Pediatrics, Yamanaguchi University School of Medicine

• OHSHIMA Toshifumi

  Meiji Cell Technology Center

• ISHII Kumiko

  Meiji Cell Technology Center

Bibliographic Information

Other Title

• 人工合成種特異抗原を用いた<I>Chlamydia trachomatis</I>抗体測定キットの臨床的検討
• 人工合成種特異抗原を用いたChlamydia trachomatis抗体測定キットの臨床的検討
• ジンコウ ゴウセイシュ トクイ コウゲン オ モチイタ Chlamydia t

Abstract

We have evaluated a new kit, PEPTIDE Chlamydia (Meiji Milk Products Co., Ltd, Tokyo), for detecting species specific antibodies to Chlamydia trachomatis with synthetic peptides as an antigen. Serum samples from women with C. trachomatis cervicitis (n=45), healthy pregnant women (n=100), and children suffering from C. pneum oniae lower respiratory tract infection (n=9) were used. We have measured the serum Ig or Ig antibodies to C. trachomatis of those sera with PEPTIDE, Sero IPALIZA Chlamydia (Savyon Diagnostics, Israel), and HITAZYME Chlamydia (Hitachi Chemical Co., Ltd, Hitachi).<BR>Serum samples with discrepant results were further analyzed by a microimmunofluorescence test and immunoblotting (western blotting). Ig and Ig serum positive rate to C. trachomatis of sera from women with C. trachomatis cervicitis were similar in three kits, that is, 91% and 80% in PEPTIDE, 89% and 82% in Sero IPALIZA, and 84% and 76% in HITAZYME, respectively. Ig and Ig serum positive rate to C. trachomatis of sera from healthy pregnant women were 18% and 9% in PEPTIDE, 12% and 15% in Sero IPALIZA, and 15% and 13% in HITAZYME, respectively. Serum antibodies to C. trachomatis in serum samples from children with C. pneumoniae infection were all negative in PEPTIDE, but falsely positive in several cases in Sero IPALIZA or HITAZYME. In sera with discrepant results, PEPTIDE gave more identical results with a micro-IF test and immunoblotting analysis than Sero IPALIZA or HITAZYME.<BR>These results indicate that PEPTIDE is an useful kit to detect more species specific antibodies to Chlamydia trachomatis than former kits.

Journal

• Kansenshogaku Zasshi

  Kansenshogaku Zasshi 72 (3), 249-257, 1998

  The Japanese Association for Infectious Diseases

Keywords

• Chlamydia trachomatis
• species-specific EIA
• synthetic peptide

Details

• CRID: 1390282680022738432
• NII Article ID: 130004112623
• NII Article ID: 10008717602
• NII Book ID: AN00047715
• DOI: 10.11150/kansenshogakuzasshi1970.72.249
• ISSN: 1884569X
• ISSN: 03875911
• NDL BIB ID: 4438409
• PubMed: 9585699
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4438409
• Web Site: https://search.jamas.or.jp/link/ui/1998173435
• Data Source:
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  • NDL
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  • PubMed
  • CiNii Articles
• Abstract License Flag: Disallowed