Evaluation of Pyuria by Means of Urinary Sediment Method

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  • 膿尿の評価における尿沈渣法と白血球直接算定法の比較
  • ノウ ニョウ ノ ヒョウカ ニ オケル ニョウチンサホウ ト ハッケッキュウ
  • A Comparison to the Counting Chamber Method

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Abstract

We compared the urinary sediment method to counting chamber method in evaluating pyuria, in order to elucidate an issue of urinary sediment method. The KOVA system®was used for counting chamber method. Three hundred and ten urine specimens from the patients who visited Fuji City Central Hospital in March, 1995, were employed. The numbers of leukocytes of each specimen was counted by means of urinary sediment method and counting chamber method, and compared. The urinary leukocyte count evaluating by means of these two methods were correlated well. However, the discrepancy of urinary leukocyte counts by means of these two methods were recognized in two specimens. In these two specimens, the leukocyte count was 1 to 4/5HPF by means of urinary sediment method, but more than 50μl by means of counting chamber methods. Significant bacteria (104cfu/ml or more), positive esterse reaction, and high pH (8.5) were recognized in both spesiemens. In addition, one of two these specimens was hypotonic (specific gravity was 1.008). Therefore, it was suggested that leukocytes were sometimes disrupted with centrifugation. These results indicate that counting chamber method is more useful urinary sediment method to detect pyuria.

Journal

  • Kansenshogaku Zasshi

    Kansenshogaku Zasshi 72 (12), 1295-1299, 1998

    The Japanese Association for Infectious Diseases

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