Molecular Typing of Methicillin-Resistant <I>Staphylococcus aureus</I> in a University Teaching Hospital

• ISHIMOTO Tsuyoshi

  Department of Clinical Microbiology and Immunology, School of Allied Health Sciences, Tokyo Medical and Dental University

• CHIDA Toshio

  Department of Clinical Microbiology and Immunology, School of Allied Health Sciences, Tokyo Medical and Dental University

• OKAMURA Noboru

  Department of Clinical Microbiology and Immunology, School of Allied Health Sciences, Tokyo Medical and Dental University

Bibliographic Information

Other Title

• 大学附属病院におけるメチシリン耐性黄色ブドウ球菌の遺伝学的解析
• Molecular Typing of Methicillin-Resistant Staphylococcus aureus in a University Teaching Hospital

Abstract

Plasmid analysis and pulsed-field gel electrophoresis (PFGE) were used to study the epidemiologic relationship among methicillin-resistant Staphylococcus aureus (MRSA) strains isolated at Tokyo Medical and Dental University Hospital. We found that 263 of 276 MRSA isolates had plasmids, which could be classified into30different patterns according to the number and plasmid molecular weight. Strains which harboured a single plasmid of approximately 13.4 Mds in molecular weight were the most numerous (55.7 % of the isolates). These strains were isolated from 14 of 17 hospital wards. The largest number of strains with this plasmid pattern (33 strains) were isolated from a single ward. PFGE typing was then performed to further confirm the relationships among these 33 strains. The PFGE banding patterns of these strains were highly similar. The antibiogram profiles of these strains werealso correlated with the PFGE pattern.<BR>Thus, the results suggest that these strains are epidemiologically related and spread throughout the ward. Combined plasmid analysis and PFGE were effective for discriminating the various MRSA isolates.

Journal

• Kansenshogaku Zasshi

  Kansenshogaku Zasshi 73 (3), 225-232, 1999

  The Japanese Association for Infectious Diseases

Keywords

• MRSA
• pulsed-field gel electrophoresis (PFGE)
• plasmid
• epidemiology

Details

• CRID: 1390001205049620992
• NII Article ID: 130004330748
• NII Article ID: 10008720128
• NII Book ID: AN00047715
• DOI: 10.11150/kansenshogakuzasshi1970.73.225
• ISSN: 1884569X
• ISSN: 03875911
• NDL BIB ID: 4687701
• PubMed: 10222668
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4687701
• Web Site: https://search.jamas.or.jp/link/ui/1999158926
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• Abstract License Flag: Disallowed