Levels of Thermostable Direct Hemolysin Production by <I>Vibrio parahaemolyticus</I> Strains Carrying both <I>tdh</I> and <I>trh</I> Genes

• SUZUKI Norihiko

  Kansai Airport Quarantaine Station

• HASHIMOTO Satoru

  Kansai Airport Quarantaine Station

• ISHIBASHI Masanori

  Osaka Prefectural Institute of Public Health

• KIM Yung Bu

  Center for Southeast Asian Studies, Kyoto University

• OKUDA Jun

  Center for Southeast Asian Studies, Kyoto University

• NISHIBUCHI Mitsuaki

  Center for Southeast Asian Studies, Kyoto University

Bibliographic Information

Other Title

• <I>tdh/trh</I>両遺伝子保有腸炎ビブリオのThermostable Direct Hemolysin産生レベルについて
• tdh/trh両遺伝子保有腸炎ビブリオのThermostable Direct Hemolysin産生レベルについて
• tdh trh リョウ イデンシ ホユウ チョウエンビブリオ ノ Thermo
• Levels of Thermostable Direct Hemolysin Production by Vibrio parahaemolyticus Strains Carrying both tdh and trh Genes

Abstract

One hundred and twenty-five strains of Vibrio parahaemolyticus carrying both the tdh and trh genes were selected from the strains isolated from the travelers with diarrhea by an hybridization test using polynucleotide probes. The levels of TDH produced by these strains and the association between the TDH levels and related chracteristics in these strains were analyzed. The TDH level varied greatly from strain to strain, but none of the levels was as high as that of the typical Kanagawa phenomenon-positive strains. The strains were classified into “TDH producer”(18 strains), “Low-level TDH producer”(85 strains), and “No TDH producer”(22 strains) based on the results of a modified Elek test and the hemolysis assay on Wagatsuma agar. The highest TDH level achieved by the “TDH produce”was twofold lower than that of the Kanagawa phenomenon-positive strains as assayed by the RPLA method.<BR>All strains possessed the toxR gene. The trhl and trh2 genes were detected in, respectively, 105 and 20 strains, and no correlation existed between the type of the trh gene and the levels of TDH produced. Considerable restriction fragment length polymorphism was observed with the tdh gene-bearing HindlIl DNA fragment in different strains, but it was not related with the TDH level.

Journal

• Kansenshogaku Zasshi

  Kansenshogaku Zasshi 71 (12), 1221-1225, 1997

  The Japanese Association for Infectious Diseases

Keywords

• Vibrio parahaemolyticus
• tdh
• trh
• toxR

Details

• CRID: 1390001205046565760
• NII Article ID: 130004330519
• NII Article ID: 10008722113
• NII Book ID: AN00047715
• DOI: 10.11150/kansenshogakuzasshi1970.71.1221
• ISSN: 1884569X
• ISSN: 03875911
• NDL BIB ID: 4369109
• PubMed: 9483883
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4369109
• Web Site: https://search.jamas.or.jp/link/ui/1998109904
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  • PubMed
  • CiNii Articles
• Abstract License Flag: Disallowed