RT-PCRおよびジゴキシゲニンプロープを用いたE型肝炎ウイルスRNA検出の比較検討 A Comparative Study of Detection of Hepatitis E Virus RNA by RT-PCR and Digoxin Probe Techniques

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Author(s)

    • 趙 素元 ZHAO Su-Yuan
    • 中国新彊維吾尓自治区人民医院 Department of Infectious Diseases, the People's General Hospital of Xinjiang
    • 〓 林〓 ZHOU Lin-Yue
    • 中国新彊維吾尓自治区人民医院 Department of Infectious Diseases, the People's General Hospital of Xinjiang
    • 周 偉一 [他] ZHOU Wei-Yi
    • 中国新彊維吾尓自治区人民医院 Department of Infectious Diseases, the People's General Hospital of Xinjiang
    • 陽 忠禮 YANG Zhong-Li
    • 中国新彊維吾尓自治区人民医院 Department of Infectious Diseases, the People's General Hospital of Xinjiang
    • 仲 英娜 ZHONG Ying-Na
    • 中国新彊維吾尓自治区人民医院 Department of Infectious Diseases, the People's General Hospital of Xinjiang

Abstract

RT-PCRおよびジゴキシゲニンプローブスポットハイブリダイゼーションによるE型肝炎ウイルス核酸検出法を開発した.急性E型肝炎の250の保存便中40.9%がRT-PCRで陽性を示した.また1例では発病後28日まで糞便中に陽性を示した.血清学的には66.7%が陽性で, ジゴキシゲニンプローブを用いたPCRでは66.6%陽性であった.シゴキシゲニンを用いたRT-PCRはE型肝炎の臨床診断および基礎研究に有用であることが示された.

Techniques for detecting hepatitis E virus nucleic acid by RT-PCR and digoxin probe spot hybridization tests were developed. The examination by RT-PCR test of 250 stored stool specimens from patients with acute hepatitis E gave a positive rate of 40.9% and was positive in one stool specimen collected 28 days after the patient had contracted the illness. Serologic examination yielded a positive rate of 66.7%, and also utilizing the DIG probe labelled with PCR product it was 66.6%. RT-PCR with the digoxin probe proved to be suitable for clinical diagnosis and basic research on hepatitis E.

Journal

  • Kansenshogaku Zasshi

    Kansenshogaku Zasshi 70(5), 485-489, 1996-05-20

    The Japanese Association for Infectious Diseases

References:  8

Codes

  • NII Article ID (NAID)
    10008723124
  • NII NACSIS-CAT ID (NCID)
    AN00047715
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    03875911
  • NDL Article ID
    3968188
  • NDL Source Classification
    ZS9(科学技術--医学--病理学・微生物学・寄生虫学・感染・免疫学・血清学・アレルギー)
  • NDL Call No.
    Z19-193
  • Data Source
    CJP  NDL  J-STAGE 
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