Spotted Fever Group Rickettsiosis and Vectors in Kanagawa Prefecture

• KATAYAMA Takashi

  Kanagawa Prefectural Public Health Laboratory

• FURUYA Yumiko

  Kanagawa Prefectural Public Health Laboratory

• YOSHIDA Yoshiya

  Kanagawa Prefectural Public Health Laboratory

• KAIHO Ikuo

  Public Health Laboratory of Chiba Prefecture

Bibliographic Information

Other Title

• 神奈川県における紅斑熱群リケッチア症および媒介マダニ
• カナガワケン ニ オケル コウハンネツグン リケッチアショウ オヨビ バイカイ
• Sotted fever group rickettsiosis and vectors in Kanawaga Prefecture

Abstract

Primer pairs for PCR were designed from the gene encoding the 17, 000-molecular-weight genus-common antigen of Rickettsia japonica, Rickettsia rickettsii, Rickettsia conorii, Rickettsia typhi and Rickettsia prowazekii. Primers R1, R2 were designed for amplifying the genomic DNA from spotted fever group (SFG) rickettsiae and epidemic typhus rickettsiae. Primers Rj5, RjlO were designed for amplifying the genomic DNA from only R. japonica. Using the primers R1, R2, about a 540-bp fragment was observed by amplifying the genomic DNA from R. japonica, R.rickettsii, R. conorii, Thai tick typhus TT-118, Rickettsia sibirica, Rickettsia rnontana, Rickettsia askari, R. typhi, R. prowazekii and Katayama strain isolated from the patient infected with SFG rickettsiae. Using the primers Rj5, RjlO, the 357-bp fragment was observed by amplifying the genomic DNA from R. japonica and Katayama strain. Therefore, the Katayama strain was identified to belong to R. japonica. With primers R1, R2 and Rj5, Rj10, 537 bp and 357 bp bands were amplified from blood of the patients infected with SFG rickettsiae in Kanagawa prefecture.<BR>These findings indicate that the causative agent of SFG rickettsiosis in these two patients was R. japonica. The ticks, Ixodes ovatus and Haernaphysalis flava, were collected by out field research in Kanagawa prefecture. With primers R1, R2 and Rj5, Rj 10, 537 bp and 357 bp were amplified from these ticks.<BR>This indicates that I. ovatus and H. flava were the vector of R. japonica in Kanagawa prefecture. Also, with the primers R1, R2, about a 540 bp fragment was amplified but with primers Rj5, Rj10, no fragments were amplified from I. ovatus and H. flava. Therefore, these ticks may have SFG rickettsiae other than R. japonica and epidemic typhus rickettsiae.

Journal

• Kansenshogaku Zasshi

  Kansenshogaku Zasshi 70 (6), 561-568, 1996

  The Japanese Association for Infectious Diseases

Keywords

• spotted fever group rickettsiosis
• Rickettisia japonica
• tick

Details

• CRID: 1390001205046052096
• NII Article ID: 130004112539
• NII Article ID: 10008702614
• NII Article ID: 10008723193
• NII Book ID: AN00047715
• DOI: 10.11150/kansenshogakuzasshi1970.70.561
• COI: 1:STN:280:BymA2cvmtlQ%3D
• ISSN: 1884569X
• ISSN: 03875911
• ISSN: http://id.crossref.org/issn/03875911
• NDL BIB ID: 3987619
• PubMed: 8741704
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I3987619
• Web Site: https://search.jamas.or.jp/link/ui/1996249128
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• Abstract License Flag: Disallowed