Pillin Structural Gene and Bacterial Adhesion to Cultured Cell of <I>Pseudomonas aeruginosa</I> Isorated from Clinical Materials

DOI Web Site PubMed 2 Citations 19 References

Bibliographic Information

Other Title
  • 臨床分離緑膿菌の線毛構造遺伝子の特徴と培養細胞に対する付着性の検討
  • リンショウ ブンリ リョクノウキン ノ センモウ コウゾウ イデンシ ノ トク
  • Pillin Structural Gene and Bacterial Adhesion to Cultured Cell of Pseudomonas aeruginosa Isorated from Clinical Materials

Search this article

Abstract

Thirty-three stocks of Pseudomonas aeruginosa which is an important etiologic agent of opportunistic infecitons were clinically isolated. The pillin structural gene pilA of the stocks were amplified by polymerase chain reaction (PCR) and classified into 3 groups; 2000 bp (16 stocks; 48.5%), 1300 bp (11 stocks; 33.3%), 550 bp (6 stocks; 18.2%). The adhesiveness of the stocks to cultured human lung cancer origin calu-1 was also determined, their adhesion rate per cell were 39.2%, 24.8%, 22.1% in average respectively. Thus clinically most common 2000 pb group is remarkably easier to adhere to calu-1. Serotypes of the strains were examined to reveal the difference of the distribution that F, G, I types were dominant in 2000 pb group, but E types were major in 1300 bp and 550 bp groups. These data sugest that the gene arrangement of pilA influences adhesiveness to cultured cell and antigenicity of bacteria.

Journal

  • Kansenshogaku Zasshi

    Kansenshogaku Zasshi 70 (12), 1271-1278, 1996

    The Japanese Association for Infectious Diseases

Citations (2)*help

See more

References(19)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top