The Bactericidal Effects of an Amphoteric Surfactant with Ethanol against <I>Mycobacterium tuberculosis</I>

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  • エタノールを添加した両性界面活性剤の結核菌に対する殺菌効果
  • エタノール オ テンカシタ リョウセイ カイメン カッセイザイ ノ ケッカクキ
  • The Bactericidal Effects of an Amphoteric Surfactant with Ethanol against Mycobacterium tuberculosis

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Abstract

Bactericidal activities of an amphoteric surfactant (12w/v% Alkyl diaminoethylglycine hydrochloride, 3w/v% Alkyl dietylenetriaminoglycole hydrochloride, Nissan Anon #300®, Inui Shouji Co., ADG) against Mycobacterium tuberculosis were examined independently or in cooperation with ethanol. The results obtained in the study are as follows:<BR>1. In the treatment with the surfactant diluted with distilled water in one hundredth, the numbers of tubercle bacilli which survived were 500cfu/0.1ml after one minute-treatment, and 25cfu/0.1ml after ten minutes-treatment. On the other hand, the surfactant containing 23v/v% ethanol decreased the bacterial numbers to seven cfu/0.1ml after one minute and less than one cfu/0.1ml after ten minutes. The numbers of the survived bacilli treated with the surfactant containing 20v/v% ethanol were 500cfu/0.1 ml after one minute-treatment, and less than one cfu/0.1ml after ten minutes though 20v/v% ethanol alone was ineffective in ten minutestreatment.<BR>2. The bactericidal activities of the surfactant against tubercle bacilli in sputum samples in which 1.65×104cfu/0.1ml of the organism were contained were also investigated. The numbers of tubercle bacilli which survived were 25cfu/0.1ml after one minute-treatment with the surfactant in one fiftieth, two cfu/0.1ml after five minutes-treatmnet and one cfu/0.1ml after ten minutes-treatment. The numbers of the organism treated with the surfactant containing 23v/v% ethanol were less than one cfu/0.1ml after five minutes.<BR>3. When the scanning electoron microscopies were performed, it was observed that the cell-surface of the organisms exposed to the surfactant in one fiftieth and the surfactant containing 23v/v% ethanol became to sticky and fusible. The morphological chang was greater in the former than in the later.<BR>Those findings indicate that the bactericidal activity of the surfactant against M. tuberculosis is enhanced by cooparation with ethanol.

Journal

  • Kansenshogaku Zasshi

    Kansenshogaku Zasshi 69 (1), 91-97, 1995

    The Japanese Association for Infectious Diseases

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