ニューキノロン薬耐性淋菌の耐性機構の解析  [in Japanese] Quinolone-Resistant Mutations Neisseria gonorrhoeae  [in Japanese]

Access this Article

Author(s)

Abstract

1991年2月から1992年1月までの間に受診した淋菌性尿道炎患者から分離され, 保存し得た淋菌のうちニューキノロン薬低感受性株10株を選び, キノロン耐性機構の解析を行った. 最初に, PCG感受性でニューキノロン薬耐性の3株を用いて, 大腸菌<I>gyrA</I>遺伝子をもつプラスミドによる淋菌の形質転換実験を行い, 次いで, PCR法により淋菌<I>gyrA</I>遺伝子上の塩基配列を決定した. 形質転換実験を行った3株のうち2株で形質転換体が得られ, その中の1株はnorfioxacin (NFLX) で8倍感性化し, この株は<I>gyrA</I>変異を有していることが推定された. PCR法による淋菌<I>gyrA</I>遺伝子上の塩基配列の検討の結果, 2株はいずれも, <I>gyrA</I>遺伝子上にSer-83 (TCC) →Phe (TTC) 変異を有しており, もう1株ではこのSer→Pheへの変異に加えて, Asp-87 (GAC) →Gly (GGC) の変異を併せもっていた. こうしたキノロン耐性淋菌の<I>gyrA</I>遺伝子上の変異は, 大腸菌など他の細菌で報告されている変異と相同性が高く, わが国におけるニューキノロン薬の使用頻度の増加がこうした耐性淋菌の蔓延と関連が深いことが推測された.

For 10 strains of <I>Neisseria gonorrhoeae</I> exhibiting decreased susceptibility to new quinolones (MIC≥0.1μg/ml) isolated and preserved from patients with gonococcal urethritis during the period from February 1991 through January 1992. We investigated the mechanism for development of resistance to new quinolones. After selecting 3 PCG-sensitive and new quinoloneresistant strains from among these strains, we first carried out transformation experiments of <I>N. gonorrhoeae</I> using a plasmid carrying the E. coli gyrA gene. We then determined the base sequence on the <I>N. gonorrhoeae gyrA</I> gene by PCR method. Of the 3 strains in which tranformation experiments were carried out, 2 strains yielded transformants, one of which was 8 times more sensitive to norfloxacin (NFLX) than the original strain, and it was assumed that this strain has a mutation in the <I>gyrA</I> gene. In our study of the base sequence on the <I>N. gonorrhoeae gyrA</I> gene using the PCR method, both strains had the mutation of Ser 83 (TCC)→Phe (TTC), and in addition to this mutation of Ser→Phe, one other strain had the mutation of Asp-87 (GAC)→Gly (GGC). This type of mutation of quinolone-resistant <I>N. gonorrhoeae</I> on the <I>gyrA</I> gene has high homology with the mutations reported for <I>Escherichia coli</I> and other strains and there appears to be a close correlation between the increasing frequency of use of the new quinolones in this country and the spread of such resistant strains of <I>N. gonorrhoeae.</I>

Journal

  • Kansenshogaku Zasshi

    Kansenshogaku Zasshi 69(5), 511-516, 1995-05-20

    The Japanese Association for Infectious Diseases

References:  17

Cited by:  9

Codes

  • NII Article ID (NAID)
    10008726091
  • NII NACSIS-CAT ID (NCID)
    AN00047715
  • Text Lang
    JPN
  • Article Type
    Journal Article
  • ISSN
    03875911
  • NDL Article ID
    3618905
  • NDL Source Classification
    SC191(感染症)
  • NDL Source Classification
    ZS9(科学技術--医学--病理学・微生物学・寄生虫学・感染・免疫学・血清学・アレルギー)
  • NDL Call No.
    Z19-193
  • Data Source
    CJP  CJPref  NDL  J-STAGE 
Page Top