新しい選択増菌培地とPCR法を用いたコレラ菌の迅速検出法の確立 Rapid Detection of Vibrio cholerae with a New Selective Enrichment Medium and Polymerase Chain Reaction

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Author(s)

    • 木田 中 KIDA Nori
    • 厚生省横浜検疫所輸入食品・検疫検査センター Center for Inspection of Imported Foods and Infectious Diseases, Yokohama Quarantine Station, Ministy of Health and Welfare
    • 鈴木 荘介 SUZUKI Sousuke
    • 厚生省横浜検疫所輸入食品・検疫検査センター Center for Inspection of Imported Foods and Infectious Diseases, Yokohama Quarantine Station, Ministy of Health and Welfare
    • 田口 文章 TAGUCHI Fumiaki
    • 北里大学医療衛生学部微生物研究室 Department of Microbiology, Kitasato University School of Allied Health Sciences

Abstract

コレラ菌と大腸菌に対する金属EDTA化合物の発育抑制効果を検討した. 試験した化合物の中で, Fe-EDTAのみが大腸菌に対してpH依存の増殖抑制を示したが, コレラ菌の発育は全く抑制しなかった. 選択的抑制剤としてFe-EDTAを添加することにより, 他のグラム陰性樟菌からコレラ菌を選択的に分離培養するための新しい増菌培地VCFbrothを開発した. VCFbrothのコレラ菌に対する増菌能と大腸菌に対する選択的抑制能は, アルカリペプトン水のそれよりも顕著であった.<BR>VCF brothを用いて6時間の増菌とPolymerasechain reactionによって, 目的とするctxのDNA断片を増幅して, コレラ菌を迅速に検出するための簡単な方法を検討し提示した. VCFbrothは, 細菌検査におけるコレラ菌の選択的増菌のための有用な培地と考えられる.

The inhibitory effect of metallic EDTA compounds on growth of <I>Vibrio cholerae</I> and <I>Escherichia coli</I> was studied. Only Fe-EDTA among the compounds tested showed pH-dependent growth inhibition on <I>E. coli</I> at pH 9.0, but no inhibition of <I>V. cholerae</I> at the same pH. By addition of Fe-EDTA as a selective inhibitor, a novel enrichment broth (tentatively designated as VCF broth) for the selective isolation and cultivation of <I>V. cholerae</I> from other Gram-negative bacilli has been developed, and the selective enrichment capacity of VCF broth for <I>V. cholerae</I> and selective inhibiting activity against <I>E. coli</I> were significantly higher than those of alkaline peptone water. A simple procedure for rapid detection of <I>V. cholerae</I> by selective enrichment for 6 hr with VCF broth and then amplification of the cholera toxin target DNA fragment by the polymerase chain reaction was presented. VCF broth may be a useful tool for the selective enrichment of <I>V. cholerae</I> in bacterial examinations.

Journal

  • Kansenshogaku Zasshi

    Kansenshogaku Zasshi 69(7), 826-834, 1995-07-20

    The Japanese Association for Infectious Diseases

References:  17

Cited by:  4

Codes

  • NII Article ID (NAID)
    10008726760
  • NII NACSIS-CAT ID (NCID)
    AN00047715
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    03875911
  • NDL Article ID
    3631648
  • NDL Source Classification
    SC171(微生物学(医))
  • NDL Source Classification
    ZS9(科学技術--医学--病理学・微生物学・寄生虫学・感染・免疫学・血清学・アレルギー)
  • NDL Call No.
    Z19-193
  • Data Source
    CJP  CJPref  NDL  J-STAGE 
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