Effect of Clarithromycin on Macrophage Functions

• Xu Guang

  First Department of Internal Medicine

• FUJITA Jiro

  First Department of Internal Medicine

• NEGAYAMA Kiyoshi

  Department of Clinical Laboratory

• OHNISHI Takayuki

  First Department of Internal Medicine

• MIYAWAKI Hiroshi

  First Department of Internal Medicine

• HOJO Satoko

  First Department of Internal Medicine

• TAKIGAWA Keiichi

  First Department of Internal Medicine

• OKADA Hiroki

  First Department of Internal Medicine

• YAMAJI Yasufumi

  First Department of Internal Medicine

• TAKAHARA Jiro

  First Department of Internal Medicine

Bibliographic Information

Other Title

• クラリスロマイシンのマクロファージ機能に対する影響
• クラリスロマイシン ノ マクロファージ キノウ ニ タイスル エイキョウ

Abstract

Recently, it has been suggested that macrolide antibiotics act as immunomodulators. In this study, we evaluated the effect of clarithromycin (CAM) on macrophage function. We used the mouse macrophage cell line, J774.1. The following direct effects of CAM on macrophage function were evaluated: chemotaxis to CAM, chemokinetic effect of CAM, and the effect of CAM on macrophage growth. In order to examine the indirect effects of CAM on macrophage functions, we preincubated macrophages with several concentrations of CAM and then removed the CAM. Thereafter, the phagocytosis of beads, cytocidal activity against Candida albicans, and chemotaxis to lipopolysaccharide were evaluated. In addition, the indirect effects of CAM on endoxan (4 mg/ml) treated macrophage phagocytosis, cytocidal activity, and chemotaxis were evaluated. CAM (at the concentration between 0.04 and 0.2 μg/ml) directly stimulated macrophage chemotaxis and chemokinesis. In addition, CAM dose-dependently stimulated the growth of macrophages. CAM pretreatment (for 4 hours at the concentrations between 0.04 and 0.2 μg/ml) stimulated macrophage phagocytosis, cytocidal activity against Candida albicans, and chemotaxis to lipopolysaccharide. In addition, CAM recovered macrophage phagocytosis, cytocidal activity, and chemotaxis which were decreased after endoxan exposure. These results suggest that CAM has direct and indirect effects on macrophage functions.

Journal

• Kansenshogaku Zasshi

  Kansenshogaku Zasshi 69 (8), 864-872, 1995

  The Japanese Association for Infectious Diseases

Keywords

• clarithromycin
• macrophage functions
• diffuse panbronchiolitis

Details

• CRID: 1390001205047239168
• NII Article ID: 130004330393
• NII Article ID: 10008722395
• NII Article ID: 10008726849
• NII Book ID: AN00047715
• DOI: 10.11150/kansenshogakuzasshi1970.69.864
• ISSN: 1884569X
• ISSN: 03875911
• NDL BIB ID: 3631709
• PubMed: 7594777
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I3631709
• Web Site: https://search.jamas.or.jp/link/ui/1996040573
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• Abstract License Flag: Disallowed