福岡における百日咳の流行 : 分離状況およびMIC分布(1990-1993)  [in Japanese] Prevalence of Pertussis in Fukuoka: Incidence and MICs of Antibioties for Bordetella pertussis Isolate  [in Japanese]

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Abstract

1990年8月から1993年3月までの30カ月間に福岡県内6病院小児科を受診した百日咳様患者を対象として, 百日咳菌分離を行った. この期間に菌検索を行った179検体から43株 (24%) の1, 3, 6型のK抗原因子を保有する百日咳菌と1株のパラ百日咳菌を分離した. 百日咳菌を分離した患者の年齢は1歳未満が22名 (51%), 1歳から10歳が18名 (42%), 成人が3名であった. これら43名中39名がDPTワクチン未接種であったが, 3名はDPTワクチン接種済みであった. 分離菌株の23薬剤に対する最小発育阻止濃度 (MIC) を求めた. MIC測定は微量液体希釈法で行った. PIPC, EM及びCPZのMIC<SUB>50</SUB> (使用菌株50%に対するMIC値) は0.005, 0.02及び0.01μg/mlであった. また, CER及びCCLのMIC<SUB>50</SUB>は各々10及び20μg/mlであり, 分離菌株はこれら薬剤に対して耐性であった. 更に従来から用いられている寒天平板希釈法も併せて実施し, 微量液体希釈法と比較検討した. 両法による使用菌株のMICは何れの薬剤も同じかやや寒天平板希釈法の方が高かった.

For bacteriological examinations of whooping cough patients, nasopharyngeal specimens were directly inoculated on the cyclodextrin solid medium (CSM) supplemented with 5 μg of cephalexin (CEX) per ml. The inoculated plates were cultured in an incubator at 35°C for 3 to 7 days. During the period from 1990 to 1993, <I>B. pertussis</I> (43 strains) and B. parapertussis (1 strain) were isolated from 145 whooping cough patients and 34 relatives. <I>B. pertussis</I> were isolated sufficiently in June to December during the year. It was suggested that during this period epidemics of whooping cough occurred. All isolates of <I>B. pertussis</I> had K antigen consisting of 1, 3, and 6.<BR>The determination of minimal inhibitory concentrations (MIC) of antibiotics to <I>B. pertussis</I> was carried out by the micro dilution technique modified from the standard methods. The Stainer-Scholte broth, supplemented with heptakis (2, 6-O-dimethyl)β-cyclodextrin, was used for dilution of the drug, and also for cultivation of bacteria. For determining susceptibility of bacteria to antibiotics, twenty seven isolates of <I>B. pertussis</I> and one of <I>B. parapertussis</I> were precultured on CSM at 35°C for 2 days. The turbidity of broth cultures was adjusted to that of McFarland no. 0.5, and diluted to the concentration of 10<SUP>6</SUP> CFU/ml. One hundred, μl of these suspensions were added to 25, μl of each antibiotic solution. After incubation for 3 days at 35°C, the turbidity of the bacteria was read macroscopically for determining the MICs. On the other hand, we compared the agar dilution MICs of 23 antibiotics to the broth microdilution MICs for 27 <I>B. pertussis</I> isolates. MICs determined by the agar dilution method were approximately the same or 2 fold of MICs determined by the broth microdilution method. Therefore the <I>in vitro</I> data from the present studies confirm the excellent inhibitory activity of piperacillin, erythromycin and cefoperazone, MICs which ranged from 0.001-0.156, 0.01-0.078 and 0.002-0.078 μg/ml, respectively. Cephaloridine and cefaclor MICs for 90% of <I>B. pertussis</I> isolates were 10 and 80 μg/ml, respectively.

Journal

  • Kansenshogaku Zasshi

    Kansenshogaku Zasshi 69(8), 878-883, 1995-08-20

    The Japanese Association for Infectious Diseases

References:  16

Cited by:  1

Codes

  • NII Article ID (NAID)
    10008726874
  • NII NACSIS-CAT ID (NCID)
    AN00047715
  • Text Lang
    JPN
  • Article Type
    Journal Article
  • ISSN
    03875911
  • NDL Article ID
    3631657
  • NDL Source Classification
    SC171(微生物学(医))
  • NDL Source Classification
    ZS9(科学技術--医学--病理学・微生物学・寄生虫学・感染・免疫学・血清学・アレルギー)
  • NDL Call No.
    Z19-193
  • Data Source
    CJP  CJPref  NDL  J-STAGE 
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