血清中に存在するAcholeplasma laidlawii膜成分類似のhuman immunodeficiency virus (HIV) 増殖促進物質  [in Japanese] A Factor to Enhance HIV Replication in Serum Bearing a Resemblance to Membranes of Acholeplasma laidlawii  [in Japanese]

Access this Article

Author(s)

    • 林 龍之介 HAYASHI Ryunosuke
    • 久留米大学医学部細菌学, 産婦人科学講座 Department of Bacteriology, Obstetrics & Gynecology, Kurume Unibersity School of Medicine

Abstract

我々は抗A抗体を用いたELISA法でヒト血清中のA物質の存在を明らかにした.本物質は<I>A. laidlawii</I>の膜成分より精製分画した物質でHIV増殖増強活性を示した.ヒト血清中のA物質はBligh-Dyer法による分画でクロロホルム層に認められた.また薄層クロマトグラフィーで分画するとRf値0.28に認められた.本物質は<I>A. laidlawii</I>の画分と同様にHIV増殖促進活性を認めたがTNF誘起能は認められなかった.さらにヒト血清によるHIV増殖促進活性は抗A抗体添加で抑制された.このことからヒト血清中の物質には<I>A. laidlawii</I>の膜成分に存在するHIV増殖促進物質と共通の活性部位が存在することが示唆された.

We found a substance A in human serum by ELISA method, using anti-A antibody. The substance A was obtained from the membranes of <I>A. laidlawii</I> and this enhanced HIV replication. This substance in human serum was detected in the chloroform phase of the Bligh-Dyer method. Rf value in thin layer chromatography was 0.28. This substance clearly showed enhancement of HIV replication as well as the activity of the membranes of <I>A. laidlawii</I>, but did not induce TNF production. Furthermore, the activity which enhances HIV replication in human serum was reduced by anti-A antibody. These results suggest that the substance derives from human serum which possesses a similar active site as those of substance derived from the membrane of <I>A. laidlawii</I>.

Journal

  • Kansenshogaku Zasshi

    Kansenshogaku Zasshi 69(12), 1389-1395, 1995-12-20

    The Japanese Association for Infectious Diseases

References:  20

Codes

  • NII Article ID (NAID)
    10008727695
  • NII NACSIS-CAT ID (NCID)
    AN00047715
  • Text Lang
    JPN
  • Article Type
    ART
  • ISSN
    03875911
  • NDL Article ID
    3644721
  • NDL Source Classification
    SC171(微生物学(医))
  • NDL Source Classification
    ZS9(科学技術--医学--病理学・微生物学・寄生虫学・感染・免疫学・血清学・アレルギー)
  • NDL Call No.
    Z19-193
  • Data Source
    CJP  NDL  J-STAGE 
Page Top