Analysis of Human Ouabainlike Compound by Micellar Electrokinetic Chromatography

• HARWOOD Steven

  Departments of Clinical Biochemistry

• ALFAZEMA Luwiza

  Medicine, St. Bartholomew's and the Royal London School of Medicine and Dentistry

• PERRETT David

  Medicine, St. Bartholomew's and the Royal London School of Medicine and Dentistry

• DAWNAY Anne

  Departments of Clinical Biochemistry

抄録

In this preliminary study we have optimised micellar electrokinetic chromatography (MEKC, a form of capillary electrophoresis) to enable the chromatographic and spectral characteristics of human ouabainlike compound (OLC) to be investigated. Sera from fifty patients were combined to form a pool (100ml) whilst urine (92.5ml) was obtained from a normal healthy volunteer. Both samples were initially concentrated and partially purified by solid phase extraction before further purification by sequential HPLC separations. Final volumes for both extracts were 100μl. MEKC was performed on a HP^3D CE instrument with voltage set at 20KV, capillary temperature at 20°C, injection time 4s, sample volume 10nl, with detection by photodiode array. A compound was found in both serum and urine that had similar elution and spectral characteristics to authentic ouabain. We conclude that MEKC is potentially a useful tool for the analysis of human OLC. (Hypertens Res 2000; 23 Suppl: S29-S32)

収録刊行物

• Hypertension Research

  Hypertension Research 23 (Supplement), S29-S32, 2000

  日本高血圧学会

キーワード

• ouabainlike compound
• ouabain
• micellar electrokinetic chromatography
• capillary electrophoresis

詳細情報

• CRID: 1390001204719936640
• NII論文ID: 10008741487
• NII論文ID: 130003456497
• NII書誌ID: AA10847079
• DOI: 10.1291/hypres.23.supplement_s29
• ISSN: 13484214
• ISSN: 09169636
• PubMed: 11016816
• Web Site: https://search.jamas.or.jp/link/ui/2001001521
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可