酸化Lp(a)測定の有用性 : 高血圧患者血清中の酸化Lp(a)について Evaluation of the presence of oxidized lipoprotein(a) in the serum of hypertensive patients

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In an attempt to assay native Lp (a), the anti-Lp (a) monoclonal antibody (161E2) was produced against synthetic antigen (Arg-Asn-Pro-Asp-Val-Ala-Pro). Characteristic properties of this 161E2 monoclonal antibody were identified to have reactivity to oxidative modification (treatment with CuCl2<sub>2</sub> and lipoxygenase), but to neither LDL, plasminogen nor native Lp (a). We developed a new ELISA method to measure Lp (a) modified by oxidative stress using this 161E2 monoclonal antibody as the capture and the labelled antibody, and conducted assay of the epitope in serum using BSA-peptide (16 peptides perl molecule of BSA) as the standard. Interestingly, hypertensive patients with complications showed a significantly higher level of oxidized Lp (a) in serum than did normotensive subjects (<i>p</i><0.01), whereas there was no significant difference in native Lp (a) between normotensive and hypertensive subjects. The <i>in vivo</i> presence of the epitope was also confirmed with use of 161E2 monoclonal antibody-based immunostaining of arteriosclerotic tissue layer by positive response. Upon analyzing peptide homology of the epitope, it was shown to have high homology (88%) to the cytoplasmic domain of human α2A-adrenergic receptor.

収録刊行物

  • 動脈硬化 = The journal of Japan Atherosclerosis Society

    動脈硬化 = The journal of Japan Atherosclerosis Society 29(3), 69-75, 2002-03-29

    一般社団法人 日本動脈硬化学会

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各種コード

  • NII論文ID(NAID)
    10008746111
  • NII書誌ID(NCID)
    AN00169257
  • 本文言語コード
    JPN
  • 資料種別
    ART
  • ISSN
    03862682
  • データ提供元
    CJP書誌  J-STAGE 
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