ミトコンドリアDNAマーカーによるトゲノコギリガザミ Scylla paramamosain 人工種苗の放流効果の立証 Mitochondrial DNA Markers Confirm Successful Stocking of Mud Crab Juveniles,(Scylla paramamosain) into a Natural Population

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Abstract

トゲノコギリガザミ人工種苗10万尾をミトコンドリアDNA-Dループ領域の変異で標識して, 1997年5月に高知県浦戸湾の25ヶ所に放流した。放流2ヶ月後の8月から翌々年12月までに漁獲された7標本群の遺伝的標識としたハプロタイプの頻度は, 29.6±4.3%を示し, 放流前と比べ有意に増加した (P<0.05) 。放流後のハプロタイプ頻度の変化から, 浦戸湾のトゲノコギリガザミ資源に占める放流人工種苗の割合を約17%と推定した。これらの放流した人工種苗は天然の個体と同様に次世代の繁殖に寄与していることが1998年のハプロタイプ頻度から示唆された。遺伝的標識を利用した人工種苗の追跡の有用性と放流による資源管理が含む問題を考察した。

The effectiveness of stocking artificially propagated mud crab, <I>Scylla paramamosain</I> juveniles was measured by releasing the juveniles tagged by variation of mitochondrial DNA (mtDNA) D-loop region. One hundred and forty nine females were collected to use for artificial propagation, at Urado Bay, Kochi Prefecture, Japan. The fourth leg of each was removed by autotomy, then one haplotype, #2 was selected for propagation. Only one female of 23 females showing this haplotype spawned about 3.35 million larvae. Thereafter, about 100, 000 juveniles grown up to 9.6 mm in average carapace width were released at 25 fishing grounds in Urado Bay during May, 1997. A total of 653 crabs were then collected at these sites by seven times of sampling made between Aug. 1997 and Dec. 1998. The frequency of haplotype #2 used as a genetic tag increased significantly after stocking from 15.4% to 29.6%. Thus, the ratio of released juveniles to all the mud crabs in Urado Bay was estimated to be 16.8%. The composition of the haplotype in the samples collected at the end of 1998 suggested that the fecundity of stocked crabs are the very same with those of natural stock. The relevance of mtDNA markers for stocking project and the problem of genetic management of aquatic organisms are discussed.

Journal

  • Aquaculture Science

    Aquaculture Science 50(2), 149-156, 2002-06-20

    Japanese Society for Aquaculture Science

References:  23

Cited by:  3

Codes

  • NII Article ID (NAID)
    10008750518
  • NII NACSIS-CAT ID (NCID)
    AN00124667
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    03714217
  • NDL Article ID
    6212482
  • NDL Source Classification
    ZR26(科学技術--農林水産--水産)
  • NDL Call No.
    Z18-761
  • Data Source
    CJP  CJPref  NDL  J-STAGE  JASI 
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