ミトコンドリアDNAマーカーによるトゲノコギリガザミ<I>Scylla paramamosain</I>人工種苗の放流効果の立証

DOI 日本農学文献記事索引 Web Site 被引用文献3件 参考文献23件

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  • Mitochondrial DNA Markers Confirm Successful Stocking of Mud Crab Juveniles, (Scylla paramamosain) into a Natural Population.

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The effectiveness of stocking artificially propagated mud crab, Scylla paramamosain juveniles was measured by releasing the juveniles tagged by variation of mitochondrial DNA (mtDNA) D-loop region. One hundred and forty nine females were collected to use for artificial propagation, at Urado Bay, Kochi Prefecture, Japan. The fourth leg of each was removed by autotomy, then one haplotype, #2 was selected for propagation. Only one female of 23 females showing this haplotype spawned about 3.35 million larvae. Thereafter, about 100, 000 juveniles grown up to 9.6 mm in average carapace width were released at 25 fishing grounds in Urado Bay during May, 1997. A total of 653 crabs were then collected at these sites by seven times of sampling made between Aug. 1997 and Dec. 1998. The frequency of haplotype #2 used as a genetic tag increased significantly after stocking from 15.4% to 29.6%. Thus, the ratio of released juveniles to all the mud crabs in Urado Bay was estimated to be 16.8%. The composition of the haplotype in the samples collected at the end of 1998 suggested that the fecundity of stocked crabs are the very same with those of natural stock. The relevance of mtDNA markers for stocking project and the problem of genetic management of aquatic organisms are discussed.

収録刊行物

  • 水産増殖

    水産増殖 50 (2), 149-156, 2002

    日本水産増殖学会

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