アミメノコギリガザミ, Scylla serrata 幼生の成長と生残に及ぼすワムシ中のn-3高度不飽和酸の影響 The Effect of N-3HUFA Content in Rotifers on the Development and Survival of Mud Crab, Scylla serrata, larvae

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Abstract

アミメノコギリガザミ, <I>Scylla serrata</I>をゾエア1 (Z1) 期から第1齢稚ガニ (C1) まで, 異なる濃度のn-3高度不飽和酸 (n-3HUFA) を含むワムシで飼育し, 成長や生残に及ぼす影響について調べた。飼育には1Lビーカーを1区2個ずつ用い, それぞれ30尾収容し, 30℃で稚ガニになるまで飼育した。ワムシ中のn-3HUFAの濃度は0.3から3.2%までの8段階とした。なお, Z3期以降無強化のアルテミアを併用した。生残率や各齢期に達するまでの日数はワムシ中のn-3HUFA含量と相関し, n-3HUFA0.8%含有区で優れた結果が得られた。それ以上の含量になると, Z5期からメガロバ (M) 期へ, あるいはM期からC1への変態失敗によるへい死が多く見られた。そのため, ワムシを主な餌料とした場合のn-3HUFA適正量は0.8%程度 (その時のEPAとDHAの内訳は0.33%および0.13%) であるものと推察された。

This experiment was conducted to determine the effect of the nutritional value of rotifers enriched with different kinds of lipids, especially n-3 highly unsaturated fatty acids (n-3HUFA), on the growth and survival of mud crab, <I>Scylla serrata</I> larvae. Rotifers were enriched with four kinds of materials including corn oil (R-E0, containing 0.3% n-3HUFA), four different levels of EPA28G (R-E10, R-E20, R-E40 and R-E80, containing 0.5%, 0.8%, 1.3% and 3.2% n-3HUFA, respectively), two different levels of single cell algae <I>Nannochloropsis oculata</I> (R-N18 and R-N42, containing 1.8% and 3.1% n-3HUFA, respectively) and Yugen (R-YG, containing 1.7% n-3HUFA) . Mud crab larvae were reared in one-liter plastic beakers at a stocking density of 30 larvae/L.<BR>The growth performances of mud crab larvae were affected by n-3HUFA levels and the ratio of docosahexaenoic acid (DHA; 22: 6n-3) and eicosapentaenoic acid (EPA; 20: 5n-3) contained in rotifers. Mud crab larvae fed rotifers containing 0.8% of n-3HUFA had a highest percentage of reaching the first crab stage. Increasing the n-3HUFA content of rotifers in excess of 0.8% caused a high rate of megalopa molting failure, and most mud crab larvae were failed to molt to megalopa when larvae were fed rotifers containing 3.1% n-3HUFA.<BR>The results suggested that n-3HUFA content in rotifers should be adjusted to 0.8% (containing 0.33% EPA and 0.13% DHA) in terms of growth performance for the mud crab, <I>Scylla serrata</I> larvae.

Journal

  • Aquaculture Science

    Aquaculture Science 50(2), 205-212, 2002-06-20

    Japanese Society for Aquaculture Science

References:  26

Cited by:  8

Codes

  • NII Article ID (NAID)
    10008750686
  • NII NACSIS-CAT ID (NCID)
    AN00124667
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    03714217
  • NDL Article ID
    6212606
  • NDL Source Classification
    ZR26(科学技術--農林水産--水産)
  • NDL Call No.
    Z18-761
  • Data Source
    CJP  CJPref  NDL  J-STAGE  JASI 
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