PCRによるホルマリン固定パラフィン包埋組織から豚サーコウイルス遺伝子の検出  [in Japanese] Detection of Porcine Circovirus Genes in Formalin-fixed, Paraffin-embedded Tissues of Pigs bv Polymerase Chain Reaction  [in Japanese]

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Abstract

1993~1999年に北海道の3養豚場 (B, C, D) において, 病理学的に豚サーコウイルス (PCV) 感染を伴う発育不良豚と診断された豚のパラフィン包埋組織材料 (35検体) から, PCRによりPCV遺伝子の検出を試みた. 検出率が高かったのはNlfとN2rプライマー組であり (85.7%), PCR産物にPCV-1には存在しない制限酵素 (<I>Ava</I> II, <I>Nsp</I> I) 切断部位が認められた. また, C農場の7検体はSa61により切断されなかったが, D農場の4検体は切断された. B農場では, SacI切断部位を有する15検体と切断されない4検体が混在していた. この結果は, 北海道には制限酵素切断パターンで識別できるPCV-2が少なくとも2タイプ存在することを示唆している.

Porcine circovirus (PCV) genes were detected in formalin-fixed pig-tissue samples embedded in paraffin. The tissues came from pigs affected by the postweaning multisystemic wasting syndrome and demonstrating pathological evidence of PCV infection. The samples were collected between 1993 to 1998 from farms B, C, and D in Hokkaido, Japan, and were examined by polymerase chain reaction (PCR). Among primers, detection rate of the Nlf and N2r primer set was highest. Of the 35 (85.7%) samples examined 30 were PCR positive. The cleavage sites recognized by restriction enzymes (<I>Ava</I> II and<I>Nsp</I> I) in these amplified PCR fragments were missing in the fragment amplified from PCV-1. The Sac I site was present in fragments amplified from the 4 samples from farm D but absent in the 7 fragments from farm C. Although 15 of the 19 samples from farm B had the Sac I site, the remaining 4 samples lacked it. These results suggest that at least 2 types of genetically different PCV-2 exist in Hokkaido.

Journal

  • Journal of the Japan Veterinary Medical Association

    Journal of the Japan Veterinary Medical Association 54(4), 260-264, 2001-04-20

    Japan Veterinary Medical Association

References:  24

Codes

  • NII Article ID (NAID)
    10008760570
  • NII NACSIS-CAT ID (NCID)
    AN00191857
  • Text Lang
    JPN
  • Article Type
    ART
  • ISSN
    04466454
  • NDL Article ID
    5754682
  • NDL Source Classification
    ZR22(科学技術--農林水産--畜産)
  • NDL Call No.
    Z18-352
  • Data Source
    CJP  NDL  J-STAGE  JASI 
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