Rapid Colorimetric Assay and Yeast Surface Display for Screening of Highly Functional Fungal Lignin Peroxidase

この論文にアクセスする

この論文をさがす

著者

抄録

Rapid screening method using color reaction was developed to quantitatively evaluate the lignin peroxidase (LiP) activity. For 2, 4-dichlorophenol (DCP) degradation activity, this method correlated well with the HPLC method. The lignin peroxidase H2 gene was isolated from <I>Phanerochaete chrysosporium</I>, a white-rot fungus. The molecular weight of the recombinant LiP was ca. 38 kDa, which corresponded to the predicted mature LiP H2 gene. We performed cloning into pYD vector for yeast cell surface display of the gene. The surface expression was checked by the rapid, colorimetric screening method. The supernatant did not change the color, but the washed yeast cells changed the color from white to red, which confirmed the enzymes were located on the cell surface. Five colonies expressing the LiP H2 gene at high levels were selected. The average amount of 2, 4-DCP degradation was about 23%. It was approximately 63% degradation level of the LiP from the wild type fungus. This screening and selection protocol is being applied to the DNA shuffling effort to screen LiPs with improved functionality and stability.

収録刊行物

  • Journal of chemical engineering of Japan

    Journal of chemical engineering of Japan 35(6), 527-532, 2002-06-01

    公益社団法人 化学工学会

参考文献:  21件中 1-21件 を表示

被引用文献:  1件中 1-1件 を表示

各種コード

  • NII論文ID(NAID)
    10008835162
  • NII書誌ID(NCID)
    AA00709658
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    00219592
  • NDL 記事登録ID
    6184328
  • NDL 雑誌分類
    ZP1(科学技術--化学・化学工業)
  • NDL 請求記号
    Z53-R395
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
ページトップへ