Coordination Behavior of O_2 and CO in a Solid Film Consisting of Hemoglobin and Maltose

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Abstract

Solid hemoglobin (Hb) films were prepared by casting from a mixed solution of human hemoglobin and maltose (wt ratio of Hb/maltose is 60/40). The behavior of O<sub>2</sub> and CO in coordinating to Hb in the films was then observed. The coordination equilibrium constants of both ligands were smaller by about an order of magnitude, and the rate constants (<i>k</i><sub>on</sub> and <i>k</i><sub>off</sub>) were smaller by about 13 orders of magnitude, than those of aqueous solutions of Hb. Interestingly, O<sub>2</sub> and CO saturation was limited to 52% and 86%, respectively, under a partial pressure of 760 Torr (1 Torr = 133.322 Pa) of each gas, in spite of the one-to-one ligand binding ratio in aqueous solutions. This is due to the restraint of the structural change from a tense to a relaxed state in the solid phase. The limited saturation of the coordination in the solid phase was found to increase to 100% when a small amount (5.9%) of water was added; a significant structural change from the tense to the relaxed states followed.

Journal

  • Bulletin of the Chemical Society of Japan

    Bulletin of the Chemical Society of Japan 68(3), 1006-1011, 1995-03-15

    The Chemical Society of Japan

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Codes

  • NII Article ID (NAID)
    10008888126
  • NII NACSIS-CAT ID (NCID)
    AA00580132
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    00092673
  • Data Source
    CJP  J-STAGE 
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