Enzyme Reactions in Microstructured Media. Subtilisin Catalysis in Alkyl Glucopyranosides Aggregates

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Author(s)

    • KUNUGI Shigeru
    • Laboratory for Biopolymer Physics, Department of Polymer Science and Engineering, Kyoto Institute of Technology
    • HAYASHI Yuuji
    • Laboratory for Biopolymer Physics, Department of Polymer Science and Engineering, Kyoto Institute of Technology
    • KOYASU Akira
    • Laboratory for Biopolymer Physics, Department of Polymer Science and Engineering, Kyoto Institute of Technology
    • TANAKA Naoki
    • Laboratory for Biopolymer Physics, Department of Polymer Science and Engineering, Kyoto Institute of Technology
    • SHIRAISHI Makoto
    • Laboratory for Biopolymer Physics, Department of Polymer Science and Engineering, Kyoto Institute of Technology

Abstract

In order to understand the effect of the structures of the surrounding medium on biocatalytic reactions, hydrolytic catalysis by subtilisin Carlsberg in a ternary system containing octyl <i>β</i>-D-glucopyranoside (<i>β</i>-OG), water, and octanol (OcOH) was studied, with special reference to the enzyme reactivity and the morphology of the surfactant aggregates. <br>First, the effect of <i>β</i>-OG and other alkyl D-glucopyranosides (AG) on the subtilisin reaction was studied in the surfactant/water binary system; all four surfactants tested were found to inhibit the subtilisin catalysis. <br>Then, a detailed phase diagram of <i>β</i>-OG/water/octanol was determined and several liquid crystalline phases were found. <br>When the subtilisin reactions were measured in several compositions in the phase diagram, the maximum of the apparent rate was observed in reversed micells as well as in the reversed hexagonal phase at a certain [water]/[surfactant] ratio (<i>w</i><sub>o</sub>). The apparent optimal <i>w</i><sub>o</sub> was calculated to be around 5—6. These results were compared with the results in another ternary system of Aerosol OT/water/isooctane.

Journal

  • Bulletin of the Chemical Society of Japan

    Bulletin of the Chemical Society of Japan 68(3), 1012-1018, 1995-03-15

    The Chemical Society of Japan

References:  28

  • <no title>

    KUNUGI S.

    Langmuir 7, 1576, 1991

    Cited by (1)

  • <no title>

    MARTINEK K.

    Eur. J. Biochem. 155, 453, 1986

    Cited by (3)

  • <no title>

    KLYACHKO N. L.

    Eur. J. Biochem. 161, 149, 1986

    Cited by (1)

  • <no title>

    MARTINEK K.

    Biochim. Biophys. Acta. 981, 161, 1989

    Cited by (2)

  • <no title>

    CHOPINEAU J.

    Eur. J. Biochem. 183, 459, 1989

    Cited by (1)

  • <no title>

    LUISI P. L.

    Reverse Micelles, 1984

    Cited by (3)

  • <no title>

    LUISI P. L.

    Angew. Chem. 24, 439, 1985

    Cited by (2)

  • <no title>

    WAKS M.

    Proteins 1, 4, 1986

    Cited by (1)

  • <no title>

    EKWALL P.

    J. Collid Interface Sci. 33, 215, 1970

    Cited by (1)

  • <no title>

    FONTELL K.

    J. Collid Interface Sci. 44, 318, 1973

    Cited by (1)

  • <no title>

    LAUGHLIN R. G.

    Surfactants 53, 1984

    Cited by (1)

  • <no title>

    VINSON P. K.

    J. Collid Interface Sci. 142, 74, 1991

    Cited by (1)

  • <no title>

    STUBBS G. W.

    Biochim. Biophys. Acta. 425, 46, 1975

    Cited by (1)

  • <no title>

    LAZO J. S.

    Anal. Biochem. 102, 68, 1980

    Cited by (1)

  • <no title>

    ROSEVEAR P.

    Biochemistry 19, 4108, 1980

    Cited by (2)

  • <no title>

    MATSUMURA S.

    J. Am. Oil Chem. Soc. 67, 996, 1990

    Cited by (9)

  • <no title>

    JEFFREY G. A.

    Acc. Chem. Res. 19, 168, 1986

    Cited by (2)

  • <no title>

    CHUNG Y. J.

    Biochim. Biophys. Acta. 985, 300, 1989

    Cited by (1)

  • <no title>

    ROSEVEAR F. B.

    J. Am. Oil Chem. Soc. 31, 628, 1954

    Cited by (5)

  • <no title>

    BENBER M. L.

    Mechanisms of Homogeneous Catalysis from Protons to Proteins Cha 12, 1971

    Cited by (1)

  • <no title>

    TAMAMUSHI B.

    Colloid Polym. Sci. 258, 174, 1980

    Cited by (1)

  • <no title>

    KABANOV A. V.

    J. Theor. Biol. 133, 327, 1988

    Cited by (1)

  • <no title>

    BRU R.

    Biochem. J. 259, 355, 1989

    Cited by (1)

  • <no title>

    VERHAERT R. M. D.

    Eur. J. Biochem. 187, 59, 1990

    Cited by (1)

  • <no title>

    RUCKENSTEIN E.

    J. Colloid Interface Sci. 139, 408, 1990

    Cited by (2)

  • <no title>

    MAESTRO M.

    J. Colloid Interface Sci. 154, 298, 1990

    Cited by (1)

  • <no title>

    CHOPINEAU J.

    Pure Appl. Chem. 64, 1757, 1992

    DOI  Cited by (1)

  • <no title>

    OLDFIELD C.

    Biochem. J. 272, 15, 1990

    Cited by (1)

Codes

  • NII Article ID (NAID)
    10008888162
  • NII NACSIS-CAT ID (NCID)
    AA00580132
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    00092673
  • Data Source
    CJP  J-STAGE 
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