Enzyme Reactions in Microstructured Media. Subtilisin Catalysis in Alkyl Glucopyranosides Aggregates

• Shigeru Kunugi

  Laboratory for Biopolymer Physics, Department of Polymer Science and Engineering, Kyoto Institute of TechnologySakyo-ku, Kyoto 606

• Yuuji Hayashi

  Laboratory for Biopolymer Physics, Department of Polymer Science and Engineering, Kyoto Institute of TechnologySakyo-ku, Kyoto 606

• Akira Koyasu

  Laboratory for Biopolymer Physics, Department of Polymer Science and Engineering, Kyoto Institute of TechnologySakyo-ku, Kyoto 606

• Naoki Tanaka

  Laboratory for Biopolymer Physics, Department of Polymer Science and Engineering, Kyoto Institute of TechnologySakyo-ku, Kyoto 606

• Makoto Shiraishi

  Laboratory for Biopolymer Physics, Department of Polymer Science and Engineering, Kyoto Institute of TechnologySakyo-ku, Kyoto 606

抄録

In order to understand the effect of the structures of the surrounding medium on biocatalytic reactions, hydrolytic catalysis by subtilisin Carlsberg in a ternary system containing octyl β-D-glucopyranoside (β-OG), water, and octanol (OcOH) was studied, with special reference to the enzyme reactivity and the morphology of the surfactant aggregates. First, the effect of β-OG and other alkyl D-glucopyranosides (AG) on the subtilisin reaction was studied in the surfactant/water binary system; all four surfactants tested were found to inhibit the subtilisin catalysis. Then, a detailed phase diagram of β-OG/water/octanol was determined and several liquid crystalline phases were found. When the subtilisin reactions were measured in several compositions in the phase diagram, the maximum of the apparent rate was observed in reversed micells as well as in the reversed hexagonal phase at a certain [water]/[surfactant] ratio (wo). The apparent optimal wo was calculated to be around 5—6. These results were compared with the results in another ternary system of Aerosol OT/water/isooctane.

収録刊行物

• Bulletin of the Chemical Society of Japan

  Bulletin of the Chemical Society of Japan 68 (3), 1012-1018, 1995-03-01

  Oxford University Press (OUP)

キーワード

• General Chemistry

詳細情報

• CRID: 1360846644034257408
• NII論文ID: 10008888162
• NII書誌ID: AA00580132
• DOI: 10.1246/bcsj.68.1012
• ISSN: 13480634
• ISSN: 00092673
• Web Site: https://academic.oup.com/bcsj/article-pdf/68/3/1012/56192624/bcsj.68.1012.pdf
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