Synthesis and Properties of High-Molecular-Weight Polypeptides Containing Tryptophan II. Copolypeptides of Tryptophan with Various Amino Acids^<1)>

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Author(s)

    • IIZUKA Yasuko
    • Department of Biochemistry and Engineering, Faculty of Engineering, Gunma University
    • OHTA Eido
    • Department of Biochemistry and Engineering, Faculty of Engineering, Gunma University
    • WAKAMATSU Kaori
    • Department of Biochemistry and Engineering, Faculty of Engineering, Gunma University
    • OYA Masanao
    • Department of Biochemistry and Engineering, Faculty of Engineering, Gunma University

Abstract

High-purity <i>N</i>-carboxyl <FONT SIZE="-2">L</FONT>-tryptophan anhydride (<FONT SIZE="-2">L</FONT>-4-indoylmethyl-2,5-oxazolidinedione) was synthesized in good yield. Copolypeptides with a random sequence of <FONT SIZE="-2">L</FONT>-trypophan (Trp) with glycine (Gly), <FONT SIZE="-2">L</FONT>-alanine (Ala), <FONT SIZE="-2">L</FONT>-<i>α</i>-amino-<i>n</i>-butyric acid (Abu), <FONT SIZE="-2">L</FONT>-<i>n</i>-valine (Nval), <FONT SIZE="-2">L</FONT>-<i>n</i>-leucine (Nleu), <FONT SIZE="-2">L</FONT>-leucine (Leu), <FONT SIZE="-2">L</FONT>-<i>γ</i>-methylglutamate (Glu(OMe)), and <FONT SIZE="-2">L</FONT>-methionine (Met) were synthesized by the copolymerization of the corresponding <i>N</i>-carboxy-<i>α</i>-amino acid anhydrides (NCA) in solution. The rate of polymerization of Trp NCA was less than that of the other amino acid NCAs. The helix contents of the formed copolypeptides, estimated by the circular dichroism (CD) spectra, were remarkably decreased at a content of about 8% of Trp involved in poly(Trp-co-Nval) (poly(Trp,Nval)), poly(Trp-co-Leu) (poly(Trp,Leu)), poly(Trp-co-Nleu) (poly(Trp,Nleu)), and poly(Trp-co-Glu(OMe)) (poly(Trp,Glu(OMe))), but was not decreased at that percentage in poly(Trp-co-Met) (poly(Trp,Met)).

Journal

  • Bulletin of the Chemical Society of Japan

    Bulletin of the Chemical Society of Japan 69(3), 791-796, 1996-03-15

    The Chemical Society of Japan

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Codes

  • NII Article ID (NAID)
    10008901090
  • NII NACSIS-CAT ID (NCID)
    AA00580132
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    00092673
  • Data Source
    CJP  J-STAGE 
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