Characterization of Collagen by Capillary Electrophoresis

Access this Article

Search this Article

Author(s)

Abstract

Characterization of pepsin-solubilized type I collagen was examined by a capillary electrophoresis using a capillary coated with <i>α</i>-dodecyl-<i>ω</i>-hydroxy poly(oxyethylene) to reduce protein adsorption and electroosmic flow. A good peak separation for each polypeptide was achieved at pH 5.6—6.5. The peaks were assigned to <i>α</i>1, <i>α</i>2, <i>β</i>11, <i>β</i>12, and <i>γ</i> by gel chromatography and reversed phase HPLC. It is found that each <i>α</i> chain signal split into several peaks, reflecting various residues of teropeptide.

Journal

  • Bulletin of the Chemical Society of Japan

    Bulletin of the Chemical Society of Japan 69(12), 3575-3579, 1996-12-15

    The Chemical Society of Japan

References:  16

  • <no title>

    ROYCE P. M.

    Connective Tissue and Its Hedtable Disorders, 1993

    Cited by (1)

  • <no title>

    MIYATA T.

    Fragrance J. 2, 109, 1989

    Cited by (1)

  • <no title>

    IKADA Y.

    J. Artif Organs 17, 446, 1988

    Cited by (1)

  • <no title>

    OGATA T.

    Biomaterial 13, 448, 1992

    Cited by (1)

  • <no title>

    FURTHMAYR H.

    Inst. Rev. Conn. 7, 64, 1976

    Cited by (1)

  • <no title>

    PONZ B.

    Eur J. Biochem. 16, 50, 1970

    Cited by (1)

  • <no title>

    LAEMMLI U. K.

    Nature 227, 680, 1970

    Cited by (126)

  • <no title>

    FUKAE M.

    J. Biol. Chem. 255, 6577, 1980

    Cited by (1)

  • <no title>

    FUKAE M.

    Biochem. Biophys. Res. Commun 71, 654, 1976

    Cited by (1)

  • <no title>

    MCCORMICK R. M.

    Anal. Chem. 60, 2322, 1988

    Cited by (6)

  • <no title>

    COBB K. A.

    Anal. Chem. 61, 2226, 1989

    Cited by (3)

  • <no title>

    WU C. -T.

    Anal. Chem. 64, 886, 1992

    Cited by (2)

  • <no title>

    TOWNS J. K.

    Anal. Chem. 63, 1126, 1991

    Cited by (5)

  • <no title>

    PIEZ K. A.

    Biochim. Biophys. Acta 53, 596, 1961

    Cited by (1)

  • <no title>

    EASTOE J. E.

    The Science and Technology of Gelatin, 1977

    Cited by (1)

  • <no title>

    OFFORD R. E.

    Nature 211, 591, 1966

    Cited by (5)

Codes

  • NII Article ID (NAID)
    10008912321
  • NII NACSIS-CAT ID (NCID)
    AA00580132
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    00092673
  • NDL Article ID
    4098903
  • NDL Source Classification
    ZP1(科学技術--化学・化学工業)
  • NDL Call No.
    Z53-B35
  • Data Source
    CJP  NDL  J-STAGE 
Page Top