Roles of 2'-Hydroxyls of Leadzyme on an RNA Cleavage

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Author(s)

Abstract

The roles of two different 2′-hydroxyls near to a catalytic site of a leadzyme (Pb<sup>2+</sup> ribozyme), CUGG<u>GA</u>GUCC, have been investigated kinetically. These roles of two different 2′-hydroxyls were clearly distinguished upon a cleavage reaction of an RNA substrate. One 2′-hydroxyl of <u>G</u> of the leadzyme in an asymmetry internal loop contributed to only the binding constant for the leadzyme with the substrates. The other 2′-hydroxyl of <u>A</u> in the asymmetry loop contributed to only the cleavage rate constant. On the basis of the results, we propose the possibility of the formation of a hydrogen bond between the 2′-hydroxyl of <u>G</u> of the leadzyme and the substrate and the binding between Pb<sup>2+</sup> ion and the 2′-hydroxyl of <u>A</u>.

Journal

  • Bulletin of the Chemical Society of Japan

    Bulletin of the Chemical Society of Japan 70(11), 2743-2747, 1997-11-15

    The Chemical Society of Japan

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Codes

  • NII Article ID (NAID)
    10008925389
  • NII NACSIS-CAT ID (NCID)
    AA00580132
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    00092673
  • NDL Article ID
    4346207
  • NDL Source Classification
    ZP1(科学技術--化学・化学工業)
  • NDL Call No.
    Z53-B35
  • Data Source
    CJP  NDL  J-STAGE 
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