Substitution for Apoprotein of Neocarzinostain by Self-Aggregate of Cholesterol-Bearing Pullulan

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Abstract

A nanoparticle of cholesterol-bearing pullulan (CHP) self-aggregates effectively complexed with a neocarzinostatin chromophore (NCS-chr) in 0.5 M (1 M = 1 mol dm<sup>−3</sup>) AcONa/AcOH (pH 4.7) at 2 °C in the dark. The complex so obtained was very colloidally stable even after 4 d at 37 °C in a weak acidic medium. The complexed NCS-chr still maintained in vitro cytotoxicity to HeLa, HepG2, and 3′-mRLh-2 cells. In all cases, the cytotoxicity of the complex was higher than that of free NCS-chr itself. When the galactose moiety was additionally conjugated to CHP, the modified complex showed high cytotoxicity to a 3′-mRLh-2 cell. This cytotoxicity of the complex was almost comparable to that of intact NCS, which is ascribed to the galactose specific receptor-mediated endocytosis of the modified complex. This is the first finding to show that the complexed NCS-chr so obtained still maintains the strong cytotoxicity in vitro, and that the self-aggregate of hydrophobized polysaccharide (HPS) can nicely substitute for the apoprotein of NCS.

Journal

  • Bulletin of the Chemical Society of Japan

    Bulletin of the Chemical Society of Japan 71(11), 2681-2685, 1998-11-15

    The Chemical Society of Japan

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Codes

  • NII Article ID (NAID)
    10008938794
  • NII NACSIS-CAT ID (NCID)
    AA00580132
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    00092673
  • NDL Article ID
    4603260
  • NDL Source Classification
    ZP1(科学技術--化学・化学工業)
  • NDL Call No.
    Z53-B35
  • Data Source
    CJP  NDL  J-STAGE 
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