Reorganization of Golgi Apparatus by Brefeldin A in the Embryonic Epidermal Cells of Xenopus laevis.

• Nishikawa Sumio

  Department of Biology, Tsurumi University, School of Dental Medicine

• Sasaki Fumie

  Department of Biology, Tsurumi University, School of Dental Medicine

抄録

The Golgi apparatus of Xenopus embryonic epidermal cells was examined by an inhibition experiment using brefeldin A (BFA), to know its target Golgi components since BFA caused rapid reorganization of the Golgi apparatus. Three different probes were used to identify the Golgi and Golgirelated components by fluorescence or electron microscopy: firstly anti-clathrin antibody for distinction of non-clathrin- and clathrincoated vesicles, secondly C₅-DMB-Ceramide as a trans-Golgi membrane marker, and thirdly anti-chondroitin 6-sulfate for identification of immature secretory granules. BFA caused rapid disorganization of the Golgi apparatus to form small vesicle clusters. Immature secretory granules did not contribute to the formation of the Golgi-derived vesicle clusters. The vesiculation of the stacked structures of the Golgi cisternae by BFA preceded the BFA-inhibition of non-clathrinand clathrin-coated vesicle formation. Thus, the results raise a question as to whether the BFA inhibition of coated vesicle formation and the vesiculation of the Golgi cisternae by BFA are different events.

収録刊行物

• Ａｃｔａ　Ｈｉｓｔｏｃｈｅｍｉｃａ　ｅｔ　Ｃｙｔｏｃｈｅｍｉｃａ

  Ａｃｔａ　Ｈｉｓｔｏｃｈｅｍｉｃａ　ｅｔ　Ｃｙｔｏｃｈｅｍｉｃａ 28 (2), 119-127, 1995

  日本組織細胞化学会

キーワード

• Brefeldin A
• Golgi apparatus
• Immunocytochemistry
• Fluorescent ceramide
• <I>Xenopus</I> epidermis

詳細情報

• CRID: 1390282679837998592
• NII論文ID: 10008976233
• NII書誌ID: AA00508022
• DOI: 10.1267/ahc.28.119
• COI: 1:CAS:528:DyaK2MXoslSmsbo%3D
• ISSN: 13475800
• ISSN: 00445991
• Web Site: http://www.jstage.jst.go.jp/article/ahc1968/28/2/28_2_119/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可