A Multicenter Study of a New <I>Helicobacter pylori</I> Selective Medium, Columbia Horse Blood Agar HP

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  • HASEGAWA Miyuki
    Chemotherapy Division, Mitsubishi Kagaku Bio-Clinical Laboratories, Inc.
  • AMANO Ayako
    Chemotherapy Division, Mitsubishi Kagaku Bio-Clinical Laboratories, Inc.
  • MURAOKA Hiroe
    Chemotherapy Division, Mitsubishi Kagaku Bio-Clinical Laboratories, Inc.
  • KOBAYASHI Intetsu
    Chemotherapy Division, Mitsubishi Kagaku Bio-Clinical Laboratories, Inc.
  • KIMOTO Mami
    Second Department of Internal Medicine, Oita Medical University
  • KATO Mototsugu
    Department of Endoscopy, Hokkaido University
  • FUJIOKA Toshio
    General Medicine Center, Oita Medical University
  • NASU Masaru
    Second Department of Internal Medicine, Oita Medical University

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Other Title
  • 新しく開発した<I>Helicobacter pylori</I>選択分離培地Columbia horse blood agar HPの有用性に関する複数施設評価
  • 新しく開発したHelicobacter pylori選択分離培地Columbia horse blood agar HPの有用性に関する複数施設評価
  • アタラシク カイハツ シタ Helicobacter pylori センタク ブンリバイチ Columbia horse blood agar HP ノ ユウヨウセイ ニ カンスル フクスウ シセツ ヒョウカ
  • A Multicenter Study of a New Helicobacter pylori Selective Medium, Columbia Horse Blood Agar HP

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Abstract

We conducted a study for the growth of and selectivity for the desired microorganisms using a newly developed selective culture medium for Helicobacter pylori, Columbia horse blood agar HP (CHBHP), at three different Japanese clinical laboratories, Hokkaido, Kanto and Kyusyu. When standard strains and clinical isolates of H. pylori were examined, the recovery of the organism on the CHBHP media was comparable to that of conventional selective and nonselective media. However, colonies were obviously larger on the CHBHP media. These media yielded the highest H. pylori positive rate for clinical specimens at all the three laboratories. The detection rate of the CHBHP media in H. pylori-positive specimens was higher than that of media commonly used at the three laboratories (98.1% to 100% vs. 88.0% to 96.2%). The CHBHP media also achieved a higher detection rate for specimens from H. pylori-infected animals. CHBHP media have an excellent growth supporting ability and selectivity originating from Columbia agar base and do not require the combined use of nonselective media for the growth and isolation of the organism, resulting in lower cost. Thus, they are useful media for the selective culture and isolation of H. pylori from clinical and animal specimens.

Journal

  • Kansenshogaku Zasshi

    Kansenshogaku Zasshi 76 (5), 341-346, 2002

    The Japanese Association for Infectious Diseases

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