書誌事項
- タイトル別名
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- Cytokine-specific activation of distinct MAPK subtype cascades in human neutrophils stimulated by G-CSF, GM-CSF and TNF.
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In order to clarify the differences of the signaling pathways utilized by G-CSF, GM-CSF and TNF, we investigated activation of MAPK subtype cascades in human neutrophils stimulated by these cytokines. G-CSF exclusively activates the MEK-ERK cascade. GM-CSF activates the MEK-ERK cascade strongly and the MKK 3/ 6-p 38 MAPK cascade weakly, whereas TNF activates the MKK 3/6-p 38 MAPK cascade strongly and the MEK-ERK cascade weakly. The potency of these cytokines to activate the MEK-ERK cascade was GM-CSF>G-CSF>TNF, whereas that to activate the MKK 3/6-p 38 MAPK cascade was TNF>GM-CSF. JNK was not tyrosine-phosphorylated by any cytokine in spite of the existence of JNK proteins in human neutrophils, whereas it was tyrosine-phosphorylated by TNF in undifferentiated and all-trans retinoic acid-differentiated HL-60 cells. Increased phosphorylation of ERK or p 38 MAPK was detected within 1-5 minutes after stimulation with each cytokine and was dependent on the concentrations of cytokines used. GM-CSF-or TNF-induced superoxide (O2-) release was inhibited by p 38 MAPK inhibitor (SB203580) in a dose-dependent manner. The results indicate that G-CSF, GM-CSF and TNF activate the overlapping but distinct MAPK subtype cascades in human neutrophils and p 38 MAPK may be involved in GM -CSF-or TNF-induced O2- release.
収録刊行物
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- 炎症
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炎症 19 (3), 157-165, 1999
日本炎症・再生医学会
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詳細情報 詳細情報について
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- CRID
- 1390001204784371072
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- NII論文ID
- 130003856515
- 10009360108
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- NII書誌ID
- AN00357213
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- ISSN
- 18844006
- 03894290
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
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- 抄録ライセンスフラグ
- 使用不可