歯肉由来線維芽細胞様細胞および骨芽細胞に及ぼすキチンの脱アセチル化の影響

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  • Influence of deacetylation of chitin on fibroblast-like cells and osteoblasts.

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In oral and maxillofacial surgery, we often require wound dressing materials to cover bone or soft tissue defects and promote healing. We previously showed that materials made of chitin are suitable for this purpose.<BR>Chitin changes its physical and biological properties according to its deacetylation level. However, no report has described the influence of the deacetylation of chitin on biocompatibility. To evaluate this issue, we performed in-vitro experimental studies using materials containing various levels of deacetylated chitin.<BR>Human gingival fibloblast-like cells (HGF) and mouse osteoblast cells (MC 3 T3-E 1) were cultured on sheets made of non-deacetylated chitin and, 35%, 50%, and 70% deacetylated chitin (DAC-0, DAC-35, DAC-50 and DAC-70, respectively). The growth of HGF and MC 3 T 3-E 1, and alkaline phosphatase (ALPase) activity of MC 3 T 3-E 1 were measured. The growth of these cells on the materials was also studied by SEM.<BR>The biocompatibility of DAC-35 with these cells was high. Levels lower or higher than 35% deacetylated chitin were less biocompatible. Especially, DAC-50 and DAC-70 suppressed the growth and differentiation of these cells.

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