Extensive Skinning of Cell Membrane Diminishes the Force-Inhibiting Effect of Okadaic Acid on Smooth Muscles of Guinea Pig Hepatic Portal Vein.

• Watanabe Masaru

  Department of Physiology, Tokyo Medical University

• Takano-Ohmuro Hiromi

抄録

The influences of cell membrane permeabilization (skinning) on the okadaic acid-induced inhibition of vascular smooth muscle contraction were studied in guinea pig hepatic portal vein. Pretreatment by 1 μM okadaic acid in the absence of Ca²⁺ suppressed subsequent submaximal Ca²⁺-induced contraction in preparations permeabilized with Staphylococcus aureus α-toxin or β-escin, but not in those treated with saponin or Triton X-100. The SDS-PAGE of elutants from the preparation suggests that the loss of the inhibitory effect of okadaic acid in preparations skinned with saponin or Triton X-100 results from the leakage of some cellular components with a molecular mass of 67 to 200 kDa.<br>

収録刊行物

• The Japanese Journal of Physiology

  The Japanese Journal of Physiology 52 (2), 141-147, 2002

  一般社団法人　日本生理学会

キーワード

• okadaic acid
• smooth muscle
• skinned preparation

詳細情報

• CRID: 1390001205041282304
• NII論文ID: 10009505526
• NII書誌ID: AA00691224
• DOI: 10.2170/jjphysiol.52.141
• COI: 1:CAS:528:DC%2BD38Xmt1ait78%3D
• ISSN: 18811396
• ISSN: 0021521X
• NDL書誌ID: 6228868
• PubMed: 12139772
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I6228868
• Web Site: https://search.jamas.or.jp/link/ui/2003011385
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可