陰電荷膜を用いた酸洗浄・アルカリ誘出によるウイルス濃縮法の開発

• 片山 浩之

  東京大学大学院工学系研究科

• 嶋崎 明寛

  国土交通省近畿地方整備局

• 大垣 眞一郎

  東京大学大学院工学系研究科

書誌事項

タイトル別名

• Development of Virus Concentration Method Using Negatively Charged Membrane by Alkaline Elution after Acid Rinse.
• イン デンカ マク オ モチイタ サン センジョウ アルカリユウシュツ ニ ヨル ウイルス ノウシュクホウ ノ カイハツ

抄録

A virus concentration method was developed utilizing the electrostatic interaction among viruses, multivalent cations and membranes. A negatively charged membrane was used to adsorb viruses from water samples through which diluted sulfuric acid (pH3.0-4.8) was then passed to remove cations from the membrane, followed by the elution of viruses with alkaline solution (pH10.5-12) or with beef extract solution (pH9.5). F-specific RNA phage Qβ and poliovirus type I were used as model viruses and pure water, tap water and artificial seawater were used as samples. The method using a negatively charged membrane with acid rinse followed by alkaline elution showed the highest recovery yields among those tested for polioviruses including the 1MDS positively charged membrane, especially when applied to seawater. This method also has a potential advantage over conventional methods of using inorganic eluant such that there will be no inhibition of the subsequent virus detection by PCR.

収録刊行物

• 水環境学会誌

  水環境学会誌 25 (8), 469-475, 2002

  公益社団法人 日本水環境学会

キーワード

• negatively charged membrane
• virus concentraiton
• acid rinse
• alkaline elution
• poliovirus
• Q<i>β</i>
• seawater
• quantitative RT-PCR

詳細情報

• CRID: 1390282680056651904
• NII論文ID: 10009548546
• NII書誌ID: AN10372439
• DOI: 10.2965/jswe.25.469
• COI: 1:CAS:528:DC%2BD38XnsVKksrg%3D
• ISSN: 18813690
• ISSN: 09168958
• NDL書誌ID: 6257570
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I6257570
• Web Site: https://www.jstage.jst.go.jp/article/jswe/25/8/25_8_469/_pdf
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可