書誌事項
- タイトル別名
-
- Determination of free hydroxyproline and proline in serum by HPLC with electrogenerated chemiluminescence detection using tris(2,2'-bipyridine)ruthenium(II).
- ルテニウム サクタイ デンカイ カガク ハッコウ ケンシュツ コウソク エキタイ クロマトグラフィー ニ ヨル ケッセイ チュウ ヒドロキシプロリン ト プロリン ノ ブンセキ
この論文をさがす
抄録
A simple method for the determination of free hydroxyproline (Hyp) and proline (Pro) in serum by HPLC with electrogenerated chemiluminescence detection using tris(2,2'-bipyridine) ruthenium (II) was developed. A serum sample was applied to a centrifugal filter (Microcon, YM-10, Millipore Co., Bedford, MA, U.S.A.) and was centrifuged (14000 rpm, 60 min, 15°C). The filtrate was injected to HPLC. The HPLC conditions were as follows: separation-eluent, 20 mM sodium acetate buffer (pH 4.6), 1 mM Cu2+, 10 mM 1-octanesulfonate-acetonitrile (99 : 1, v/v); flow rate, 0.5 ml/min; column, Capcell Pak C18 (4.6 × 150 mm, Shiseido Co., Ltd); detection-reagent solution, 0.3 mM tris(2,2'-bipyridine)ruthenium chloride, 10 mM sulfuric acid, flow rate: 0.3 ml/min, electrogenerated chemiluminescence detector, ECR-2000 (Comet Co., Ltd); controlled current oxidation as 80 μA. Calibration curves plotted the peak area versus Hyp and Pro concentrations. The assay was linear in the concentration range of 0.3∼10000 and 0.6∼5000 nmol/ml serum for Hyp and Pro, respectively. The recovery was 101% (10 nmol/ml) for Hyp and 103% (50 nmol/ml) for Pro. The intera-assay coefficient of variation was less than 5% for both Hyp and Pro in the linear concentration range. The method was successfully applied to the determination of aged human serum without prior derivatization.
収録刊行物
-
- 分析化学
-
分析化学 51 (10), 953-957, 2002
公益社団法人 日本分析化学会
- Tweet
詳細情報 詳細情報について
-
- CRID
- 1390282679029194752
-
- NII論文ID
- 10009959272
-
- NII書誌ID
- AN00222633
-
- COI
- 1:CAS:528:DC%2BD38XotFKntbs%3D
-
- NDL書誌ID
- 6316251
-
- ISSN
- 05251931
- http://id.crossref.org/issn/05251931
-
- 本文言語コード
- ja
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可