Osmotic and Non-osmotic Induction of Somatic Embryogenesis by Sucrose at High Concentrations in Daucus carota L
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In the detached cotyledons of carrot, <i>Daucus carota</i> L., somatic embryogenesis was induced by 2-week treatment with sucrose at 0.29-0.73M both in the epidermal cells (type-1) and the callus-like cells generated at the cut end of the cotyledons (type-2). Type-2 embryos were produced predominantly, after the treatment with 0.29-0.44M sucrose and type-1 embryos after treatment with 0.58-0.73M sucrose. Sucrose and glucose were more effective than mannitol and sorbitol for the induction of somatic embryogenesis. Furthermore, both type-1 and type-2 embryos were induced by sucrose and glucose, but only type-2 embryos by mannitol and sorbitol. These results suggest that sucrose does not work as a mere osmoticum.
- Plant Biotechnology
Plant Biotechnology 17(2), 155-158, 2000-06-01
Japanese Society for Plant Cell and Molecular Biology