Osmotic and Non-osmotic Induction of Somatic Embryogenesis by Sucrose at High Concentrations in Daucus carota L

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Abstract

In the detached cotyledons of carrot, <i>Daucus carota</i> L., somatic embryogenesis was induced by 2-week treatment with sucrose at 0.29-0.73M both in the epidermal cells (type-1) and the callus-like cells generated at the cut end of the cotyledons (type-2). Type-2 embryos were produced predominantly, after the treatment with 0.29-0.44M sucrose and type-1 embryos after treatment with 0.58-0.73M sucrose. Sucrose and glucose were more effective than mannitol and sorbitol for the induction of somatic embryogenesis. Furthermore, both type-1 and type-2 embryos were induced by sucrose and glucose, but only type-2 embryos by mannitol and sorbitol. These results suggest that sucrose does not work as a mere osmoticum.

Journal

  • Plant Biotechnology

    Plant Biotechnology 17(2), 155-158, 2000-06-01

    Japanese Society for Plant Cell and Molecular Biology

References:  8

Cited by:  2

Codes

  • NII Article ID (NAID)
    10010146490
  • NII NACSIS-CAT ID (NCID)
    AA11250821
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    13424580
  • NDL Article ID
    5402307
  • NDL Source Classification
    ZR3(科学技術--生物学--植物)
  • NDL Call No.
    Z54-J126
  • Data Source
    CJP  CJPref  NDL  J-STAGE 
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