Agrobacterium-Mediated Transformation and Regeneration of Pharbitis nil.
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- ONO Michiyuki
- Biotechnology Institute, Akita Prefectural University
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- SAGE-ONO Kimiyo
- Institute of Biological Sciences, University of Tsukuba
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- KAWAKAMI Michihiro
- Institute of Biological Sciences, University of Tsukuba
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- HASEBE Masako
- Biotechnology Institute, Akita Prefectural University
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- UEDA Kenji
- Biotechnology Institute, Akita Prefectural University
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- MASUDA Kiyoshi
- Graduate School of Agriculture, Hokkaido University
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- INOUE Masayasu
- Biotechnology Institute, Akita Prefectural University
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- KAMADA Hiroshi
- Institute of Biological Sciences, University of Tsukuba
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抄録
A reliable method for the transformation of Pharbitis (Ipomoea) nil is described for the first time. Somatic embryos derived from immature zygotic embryos were used as starting material. These were transformed with Agrobacterium tumefaciens strain GV3101 (pMP90), which harbored the plasmid pIG121Hm that included genes for β-glucuronidase (GUS), kanamycin resistance and hygromycin resistance. Infected embryos were induced to undergo secondary embryogenesis and kanamycin-resistant embryos were selected and allowed to develop into plantlets. All transgenic plants were morphologically normal and fertile. Stable integration and expression of the transgene for GUS were confirmed by histochemical and Southern blotting analysis. Expression of the intron-containing reporter gene for GUS under control of the 35S promoter of cauliflower mosaic virus (CaMV 35S) was detected after staining. Southern hybridization confirmed the stable inheritance of the transgene for GUS.
収録刊行物
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- Plant Biotechnology
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Plant Biotechnology 17 (3), 211-216, 2000
日本植物バイオテクノロジー学会
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詳細情報 詳細情報について
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- CRID
- 1390001204329616384
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- NII論文ID
- 10010146695
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- NII書誌ID
- AA11250821
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- COI
- 1:CAS:528:DC%2BD3cXmsVeqtLg%3D
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- ISSN
- 13476114
- 13424580
- http://id.crossref.org/issn/13424580
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- NDL書誌ID
- 5513367
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可