低分子デキストランによる浸透圧性腎症回復期の病理学的研究

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  • 木村 茂三
    慶応義塾大学医学部泌尿器科学教室 慶応義塾大学医学部病理学教室

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タイトル別名
  • Pathological study of repair of experimental osmotic nephrosis caused by low molecular dextran
  • テイブンシ デキストラン ニ ヨル シントウアツセイジンショウ カイフクキ ノ ビョウリガクテキ ケンキュウ

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Wistar rats weighing about 200 g were employed. Thirty rats were maintained, given no fluid for 72 hrs. Then, 60 ml /kg of low molecular weight dextran was injected intraperitoneally. The rats were sacri-ficed 2, 3, 4, 7, 9, 11 and 13 days after injection and the process of repair of osmotic nephrosis caused to low molecular weight dextran was investigated by light and electron microscopy and alcohol PAS staining. Electron micrographs of 12 hours after administration of this material, reported in previous paper, were used as to a control. The results were summarized as follows 1) osmotic nephrosis caused by low molecular weight dextran recovered to normal histology about 2 weeks after intraperitoneal injection. 2) dextran granules in the lumina of the proximal convoluted tubules were absorbed via brush border by pinocytosis and were transported to the apical tubular invaginations of the apical cell membrane. Bulbous expansions at the bases of the apical tubular invagination became filled with the dextran granule and were then pinched off from the cell membrane to form apical tubular vesicles and apical vacuols. They formed large vacuoles fusing with lysosomes. In cases of 12 hours after low molecular weight dextran administrations, dextran granules were diffusely dispersed throughout the tubular cells and various cell organellars were extremely dislocated by absorbed dextran and water. In 5 to 7 days administration, large vacuoles partially covered with limiting membrane are formed in the cells. Then, the vacuoles were divided in small fragments. These fragmented vacuoles located at the basal area of epithelial cells. This process is thought to be a course of digestion of dextran granules by lysosome. The residual large vacuoles were excreted into the tubular lumen through cytoplasm and cell membrane. The route of dextran transportation via interstitium or peritubular capillaries was not determined in this study.

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