Development of Highly-sensitive Fructosyl-valine Enzyme Sensor Employing Recombinant Fructosyl Amine Oxidase

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  • SAKAGUCHI Akane
    Department of Biotechnology, Tokyo University of Agriculture and Technology
  • TSUGAWA Wakako
    Department of Biotechnology, Tokyo University of Agriculture and Technology
  • FERRI Stefano
    Department of Biotechnology, Tokyo University of Agriculture and Technology
  • SODE Koji
    Department of Biotechnology, Tokyo University of Agriculture and Technology

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Abstract

<p>We recently isolated a novel fructosyl-amine oxidase (FAOD) from the soil bacterium Arthrobacter sp. FV 1-1, cloned the gene encoding FAOD and succeeded in the recombinant production. This bacterial FAOD showed high specificity towards fructosyl valine (FV), a proteolytic product of hemoglobin A1c (HbA1c), but was almost completely inactive towards ε-fructosyl-lysine (ε-FK), a proteolytic product of glycated albumin. By utilizing recombinant FAOD expressed in E. coli, an FAOD sensor based on hydrogen peroxide detection was constructed. The sensor showed FV specificity, with a minimum detectable concentration of 1 μM. The sensitivity was 7.1 mAM-1cm-2, which is 17 times higher than that of the previously reported FAOD-based enzyme sensors. Because the bacterial FAOD-based sensor could detect FV concentration and discriminate FV in the presence of ε-FK, it has the great potential for use in the measurement of HbA1c in whole blood samples.</p>

Journal

  • Electrochemistry

    Electrochemistry 71 (6), 442-445, 2003-06-05

    The Electrochemical Society of Japan

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