Purification and cDNA Sequencing of Vitellogenin of the Wild Silkworm,Antheraea pernyi

• Liu Chao Liang

  Laboratory of Silkworm Genetics and Pathology, Faculty of Textile Science and Technology, Shinshu University

• Kajiura Zenta

  Laboratory of Silkworm Genetics and Pathology, Faculty of Textile Science and Technology, Shinshu University

• Shiomi Kunihiro

  Laboratory of Silkworm Genetics and Pathology, Faculty of Textile Science and Technology, Shinshu University

• Takei Ryuzo

  Nagano Women's Junior College

• Nakagaki Masao

  Laboratory of Silkworm Genetics and Pathology, Faculty of Textile Science and Technology, Shinshu University

書誌事項

タイトル別名

• Purification and cDNA Sequencing of Vitellogenin of the Wild Silkworm, <i>Antheraea pernyi</i>

抄録

We purified vitellin (ApVn) and vitellogenin (ApVg) from Antheraea pernyi by a combination of gel permeation chromatography, anion-exchange chromatography, and hydrophobic chromatography. Our results showed that the molecular size of the ApVg heavy chain determined by SDS-PAGE was approximately 210 kDa. ApVn and ApVg each consisted of only a large subunit, suggesting that ApVg should be classified in group 2 of the insect vitellogenin family. We analyzed the N-terminal amino acid sequence of ApVg and the terminal amino acid sequences of four lysyl endopeptidase-degraded fragments of ApVg. The nucleotide sequence was determined using overlapping cDNA fragments generated from reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) reactions. The ApVg cDNA was 5720 nucleotides long and coded for the entire subunit, which consisted of 1778 amino acids. The molecular weight of the predicted polypeptide was 200, 000. There is no RXRR motif, which is the cleavage site between the small and large subunits in the previtellogenins of the silkworm, Bombyx mori, the mosquito, Aedes aegypti, the sawfly, Athalia rosae, and the boll weevil, Anthonomous grandis. Two polyserine regions were found in the deduced amino acid sequence.

収録刊行物

• Ｊｏｕｒｎａｌ　ｏｆ　Ｉｎｓｅｃｔ　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ　ａｎｄ　Ｓｅｒｉｃｏｌｏｇｙ

  Ｊｏｕｒｎａｌ　ｏｆ　Ｉｎｓｅｃｔ　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ　ａｎｄ　Ｓｅｒｉｃｏｌｏｇｙ 70 (2), 95-104, 2001

  社団法人 日本蚕糸学会

キーワード

• <i>Antheraea pernyi</i>
• vitellogenin
• vitellin
• RXRR motif
• polyserine

詳細情報

• CRID: 1390282680170927360
• NII論文ID: 130003852099
• NII論文ID: 10010586506
• NII書誌ID: AA11558849
• DOI: 10.11416/jibs2001.70.95
• ISSN: 18847978
• ISSN: 13468073
• NDL書誌ID: 5952184
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I5952184
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可