Study of the Culture of Human Odontoclastsin Serum Obtained from Low Calcium-Diet Rabbits

• Ogasawara Yasushi

  Department of Pediatric Dentistry, Fukuoka Dental College

Bibliographic Information

Other Title

• 低カルシウム食飼育ウサギ血清によるヒト破歯細胞培養に関する研究

Abstract

Odontoclasts are morphologically quite similar to osteoclasts but details on the difference of both cells is still not clear. There have also been no report on the cultivation of human odontoclasts. The purpose of this study was to cultivate the cells obtained from the resorbed root surfaces of the human deciduous teeth with the serum obtained from low calcium-diet rabbits, One hundred and fifty-two human deciduous teeth in physiologic root resorption were used in this study. The cells were cultivated on bone slices with serum obtained from normal-diet rabbits, serum obtained from low calciumdiet rabbits, or serum obtained from low calcium-diet rabbits containing 10-8mol salmon calcitonin. After the cultivation, tartrate-resistant asid phosphatase (TRAP) activity was detected using a light microscope. The resorption lacunae on bone slices were examined with a scanning electron microscope.

Journal

• The Japanese Journal of Pediatric Dentistry

  The Japanese Journal of Pediatric Dentistry 38 (3), 605-614, 2000

  The Japanese Society of Pediatric Dentistry

Keywords

• Odontoclast
• Human deciduous teeth
• Low calcium-diet
• Cell culture

Details

• CRID: 1390282679662250624
• NII Article ID: 130004634033
• NII Article ID: 10010638119
• NII Book ID: AN00116228
• DOI: 10.11411/jspd1963.38.3_605
• ISSN: 05831199
• Web Site: https://search.jamas.or.jp/link/ui/2001020146
• Text Lang: ja
• Data Source:
  • JaLC
  • CiNii Articles
• Abstract License Flag: Disallowed