Epitope Distribution of Randomly Established Monoclonal Antibodies against Human Type II DNA Topoisomerases

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Author(s)

Abstract

The epitopes of about 100 monoclonal antibodies against human type II DNA topoisomerase were mapped along the enzyme molecules. Although they were randomly and independently established, epitope sites were unevenly distributed the toward N-terminal or C-terminal region. We suggest that the central catalytic domain is hidden inside the molecule and inaccessible to the antigen recognition sites. Using antibodies, we demonstrate the distinct localization of isoforms of Topo II in cultured cells. Some particularly useful antibodies are listed.

Journal

  • The Journal of Biochemistry

    The Journal of Biochemistry 132(3), 409-416, 2002-09-01

    The Japanese Biochemical Society

References:  17

Cited by:  1

Codes

  • NII Article ID (NAID)
    10010651385
  • NII NACSIS-CAT ID (NCID)
    AA00694073
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    0021924X
  • NDL Article ID
    6291252
  • NDL Source Classification
    ZR2(科学技術--生物学--生化学)
  • NDL Call No.
    Z53-B472
  • Data Source
    CJP  CJPref  NDL  J-STAGE 
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