ヒト歯髄のリンパ管に関する酵素組織化学的研究

DOI 被引用文献3件 参考文献32件

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  • Lymphatic vessels in human dental pulp:An enzymehistochemical study.

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In this study, we used an enzymehistochemical method to differentiate between lymphatic vessels and blood vessels in human dental pulp. Non-caries, human, impacted third molars from individuals 18-25 years old were obtained immediately after extraction and frozen 10 um sections were cut with a cryostat. Sections were fixed and double-stained with 5′-Nase and ALPase and then examined under a light microscope (LM), scanning electron microscope (SEM), and transmission electron microscope (TEM).<BR>The product of 5′-Nase activity was detected as a dark brown precipitate of lead sulfide on the walls of lymphatic vessels, while blood vessels were blue by ALPase reaction under LM. ALPase reaction was also observed around the lymphatic vessels, but the significance of this finding is unclear. The lead-stained reaction product of 5′-Nase activity in the lymphatic walls was also visualized by backscattered electron imaging in SEM. By TEM examination, typical ultrastructural characteristics of lymphatic walls, as have been reported in many tissues, were observed in the 5′-Nase-reactive vessels. Thick lymphatic vessels were located mainly in the center of the pulp and thin vessels were found both in the center and periphery of the pulp.<BR>However, we did not observe lymphatic vessels in the odontoblast layer in the present study.<BR>These results suggest that the 5′-Nase-ALPase double-staining method is useful for studying the fine distribution and three-dimensional architecture of lymphatic vessels in human dental pulp.

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