Effects of Ghrelin on Growth Hormone Secretion from Cultured Adenohypophysial Cells in Cattle
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- HASHIZUME TSUTOMU
- Faculty of Agriculture, Iwate University
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- HORIUCHI MAMI
- Faculty of Agriculture, Iwate University
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- TATE NAOMI
- Faculty of Agriculture, Iwate University
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- NONAKA SUMIE
- Faculty of Agriculture, Iwate University
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- KOJIMA MASAYASU
- Institute of Life Sciences, Kurume University
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- HOSODA HIROSHI
- National Cardiovascular Center Research Institute
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- KANGAWA KENJI
- National Cardiovascular Center Research Institute
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Abstract
To clarify the direct effects of ghrelin on growth hormone (GH) release from anterior pituitary (AP) cells in cattle, GH-releasing effects of human ghrelin (hGhrelin) and rat ghrelin (rGhrelin) on bovine AP cells were compared with those of GH-releasing hormone (GHRH) in vitro. The AP cells were obtained from Holstein steers and were incubated for 2 h with the peptides after incubating in DMEM for 3 days. hGhrelin and rGhrelin significantly stimulated GH release from the cultured cells at doses from 10-10 to 10-7 M and from 10-9 to 10-7 M, respectively (P<0.05). The rates of increase in GH at 10-10, 10-9, 10-8 and 10-7 M hGhrelin were 26, 26, 59 and 100% compared with controls, respectively, and those of increase in GH at 10-9, 10-8 and 10-7 M rGhrelin were 58, 74 and 106%, respectively. GHRH significantly increased GH concentrations in cultured media at a dose as low as 10-13 M compared with the control (P<0.05). When hGhrelin (10-8 M) and GHRH (10-8 M) were added together, the release of GH induced by both peptides was significantly greater than that by hGhrelin alone (P<0.05), and tended to be greater than that by GHRH alone. Somatostatin (SS, 10-7 M) significantly blunted GH release induced by hGhrelin (10-8 M) and GHRH (10-8 M)(P<0.05). In the presence of SS, the percent increase in GH released with hGhrelin plus GHRH was 42% and 14% greater than that by either hGhrelin or GHRH alone, respectively (P<0.05). These results show that ghrelin directly stimulates the release of GH from anterior pituitary cells, and that SS modifies ghrelin-stimulated GH release in cattle.
Journal
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- Endocrine Journal
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Endocrine Journal 50 (3), 289-295, 2003
The Japan Endocrine Society
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Details 詳細情報について
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- CRID
- 1390001206299519360
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- NII Article ID
- 130004443042
- 10011210938
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- NII Book ID
- AA10901436
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- COI
- 1:CAS:528:DC%2BD3sXntVCmtrg%3D
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- ISSN
- 13484540
- 09188959
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- PubMed
- 12940457
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- Text Lang
- en
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- Data Source
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- JaLC
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed